Hec1 Kinetochore Analysis
Hec1 Kinetochore Analysis - Related Videos
05:35
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Cited by 4 •
2016
Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).
12:28
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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Cited by 11 •
2015
This manuscript describes the detection of sumoylation and ubiquitination of kinetochore proteins, Ndc10 and Ndc80, in the budding yeast Saccharomyces cerevisiae.
13:59
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
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Cited by 1 •
2012
The kinetochore is where the SAC initiates its signal monitoring the mitotic segregation of the sister chromatids. A method is described to visualize the recruitment and turnover of one of the kinetochore proteins and its coordination with the chromosome motion in Drosophila embryos using a Leica laser scanning confocal system.
08:13
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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2025
This protocol details two methods of yeast cell cycle arrest and optional release, and elaborates on the use of fluorescence microscopy to study cell cycle-dependent processes in S. cerevisiae.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
FAQs
Hec1 functions within the Ndc80 complex to help establish and regulate the connection between kinetochores and spindle microtubules. This connection must remain sufficiently stable to support chromosome alignment while permitting accurate segregation. Measuring attachment behavior therefore provides a way to evaluate whether kinetochore function is properly coordinated during cell division.
Hec1 should be interpreted as part of the Ndc80 complex rather than as an isolated kinetochore component. Its role in microtubule attachment reflects participation in this larger complex, which contributes to kinetochore assembly and attachment regulation. Consequently, altered Hec1 behavior can provide information about broader kinetochore organization and function.
These measurements address different aspects of kinetochore biology. Localization indicates where Hec1 is positioned, abundance reflects its presence at relevant levels, and interaction analysis examines its molecular associations. Considering these features together can help distinguish changes in kinetochore assembly from changes affecting microtubule attachment stability or Hec1-dependent function.
A study can examine Hec1 localization, quantify its abundance, assess its interactions, or introduce a functional perturbation. The resulting observations are then related to kinetochore assembly, spindle microtubule attachment, chromosome alignment, or segregation. Using complementary measurements helps connect molecular changes with the cellular consequences observed during division.
The approach is useful when researchers need to investigate how kinetochores support chromosome behavior during cell division. In cell biology, it can clarify kinetochore assembly and attachment regulation. In cancer research, it can help examine defects associated with chromosome missegregation, aneuploidy, and compromised genome stability.
Defective connections may be associated with impaired chromosome alignment or inaccurate chromosome segregation. These cellular errors can produce chromosome missegregation and aneuploidy, meaning abnormal chromosome number, and may compromise genome stability. Hec1 kinetochore analysis links observations at the attachment site with these broader consequences of faulty cell division.