Mouse Hippocampal Neurons
Mouse Hippocampal Neurons - Related Videos
03:48
A Technique to Culture Mouse Hippocampal Neurons
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2025
The video demonstrates a method for culturing neurons from murine hippocampi. Initially, the hippocampi undergo enzymatic digestion to break down the extracellular tissue matrix. Subsequently, the digested tissue is mechanically disrupted to isolate individual neurons. Finally, these neurons are cultured on glial feeder cells.
15:40
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
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Cited by 63 •
2011
This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.
04:54
Imaging Hippocampal Neuronal Activation In Mouse Brain Sections
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2025
Source: Provenzano, G., et al. Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders. J. Vis. Exp. (2015)This video demonstrates immunohistochemical imaging to visualize hippocampal neuronal activation. Stained brain sections reveal darker regions under a bright-field microscope, indicating activated neurons.
03:05
Photostimulation and Whole-Cell Patch Clamp Recordings of Neurons in Mouse Hippocampal Slices
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2025
The video demonstrates a protocol to study synaptic interactions and neuronal responses in a transfected mouse hippocampal brain slice. It involves illuminating long-range sensory neurons to trigger action potentials and recording the resulting excitatory post-synaptic potentials in the recorded neuron using the whole-cell patch-clamp technique.
02:19
Two-Photon Microscopy of Astrocytes and Excitatory Neurons in a Cleared Mouse Hippocampal Tissue
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2025
Source: Refaeli, R., et al., Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains. J. Vis. Exp. (2022) The video demonstrates the preparation of a chamber and the neuroimaging of cleared mouse hippocampal tissue using two-photon microscopy to visualize the spatial interactions between fluorescently labeled astrocytes and excitatory neurons.
FAQs
Signal flow depends on the coordinated roles of dendrites, axons, and synapses. Dendrites receive incoming information, axons transmit outputs, and synapses provide sites for communication between neurons. Examining these structures in cultured cells allows researchers to relate neuronal morphology to electrical and chemical signaling in controlled laboratory studies.
Changes in synaptic strength provide a cellular basis for neuronal plasticity, meaning that communication can be modified by activity. In mouse hippocampal neurons, studying these changes helps researchers connect synaptic function with the biological processes that support learning, memory, and spatial information processing, without treating neuronal signaling as a fixed property.
Morphological studies examine cellular structure, while electrophysiological studies assess neuronal electrical activity. Imaging can reveal features of cell shape and organization, whereas electrophysiological analysis addresses signaling behavior. Using these approaches together helps determine whether changes observed in neuronal form are accompanied by altered activity or synaptic function.
A study may begin with hippocampal neuron isolation and culture, followed by examination of neuronal development, morphology, electrical activity, or synaptic function. Researchers then apply imaging, molecular assays, or drug treatments according to the question being tested. This workflow connects cellular observations with specific mechanisms of neuronal communication or damage.
These approaches address different levels of neuronal biology. Imaging can characterize development and morphology, molecular assays can investigate cellular components or responses, and drug treatments can test how altered conditions affect neuronal signaling or damage. Combining the methods gives a broader view of how cellular processes influence neuronal function.
Cultured mouse hippocampal neurons are useful when researchers need to examine disease-related or toxic effects at the cellular level. Their responses can be assessed through morphology, electrophysiological activity, synaptic function, or molecular measurements. Findings from these experiments help connect changes in neural signaling with broader biological and behavioral outcomes.