Mouse Kidney Perfusion
Mouse Kidney Perfusion - Related Videos
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
FAQs
Controlled pressure helps drive perfusion solution through the renal vasculature after cannulation of the heart or major vessels. This circulation supports more uniform removal of blood and delivery of the solution throughout the kidneys. Consistent flow is important because incomplete rinsing or fixation can create uneven tissue preservation, affecting the reliability of histological sections, immunostaining, and molecular analyses.
A rinsing solution primarily supports removal of blood from the renal vasculature, whereas a fixation solution preserves kidney structure for later analysis. The intended outcome determines which type of perfusion is appropriate. Distinguishing these purposes helps researchers prepare tissue either for cleaner biological measurements or for structural evaluation in histology and immunostaining.
Reducing blood contamination improves the consistency and interpretability of kidney samples. Residual blood can interfere with observations of tissue structure and with analyses that depend on staining or molecular measurements. Perfusion also limits postmortem changes, helping preserve features relevant to renal biology, pathology, toxicology, and investigations of kidney injury or disease progression.
The procedure generally includes establishing access by cannulating the heart or a major vessel, connecting the delivery system, and circulating a selected perfusion solution through the renal vasculature under controlled pressure. The process continues until the intended rinsing or fixation outcome is achieved. Careful control of these stages supports uniform tissue preparation for downstream analysis.
Researchers may select this technique when kidney samples must be prepared for histological sections, immunostaining, or molecular studies with reduced blood contamination. It is relevant to renal biology, pathology, toxicology, and investigations of kidney injury, disease progression, or treatment responses. Its value is greatest when consistent tissue preservation improves comparisons among experimental samples.
The method can produce cleaner and more consistently preserved kidney tissue, which supports clearer structural assessment and more reliable staining or molecular analysis. These improvements help researchers evaluate pathological changes, characterize injury, examine disease progression, and assess responses to treatments. In this way, tissue preparation strengthens the biological interpretation of renal experiments.