Mouse Neuro Stem Cell Solution
Mouse Neuro Stem Cell Solution - Related Videos
08:29
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
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Cited by 15 •
2008
Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.
08:33
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
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Cited by 14 •
2011
The transplantation of mouse neural stem cells (NSCs) into the spinal cords of mice with established demyelination is detailed. The preparation of NSCs, the laminectomy of thoracic vertebra 9 (T9), and transplantation of NSCs is outlined along with the pre- and post-operative care of the mice.
09:52
Isolation of Mouse Salivary Gland Stem Cells
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Cited by 41 •
2011
An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
27:44
Culture of Mouse Neural Stem Cell Precursors
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Cited by 38 •
2007
This video describes the method used for isolation of neuroprecursors from the developing cortex of embryonic mice. The procedure for removing embryos from the uterus, dissecting the cortical tissue, and digesting the isolated cerebral cortex is shown.
FAQs
Defined culture conditions are used to support neural stem cell survival and self-renewal while limiting the variables present in a whole-animal environment. Researchers can then alter experimental conditions and examine whether cells continue proliferating or begin differentiating. This controlled setting helps connect a specific treatment or signaling change with a measurable cellular response.
The model provides access to neural stem cells in a controlled in vitro environment, making it easier to examine cellular processes linked to neural development and signaling. Because experiments can focus on defined conditions rather than the full complexity of an animal, researchers can evaluate cell behavior and treatment responses more directly before considering broader biological implications.
Researchers can assess changes in proliferation and differentiation after exposing the cells to experimental conditions. Proliferation indicates how the cell population responds in terms of continued growth, whereas differentiation reflects changes toward specialized neural cell states. Examining both outcomes helps reveal whether a condition supports stem cell maintenance, alters development, or produces another biological response.
The workflow begins with neural stem cells isolated from mice and maintained under defined culture conditions that support survival and self-renewal. Researchers then introduce the condition, treatment, or experimental comparison of interest and evaluate resulting changes in proliferation or differentiation. This sequence provides a practical framework for testing cellular mechanisms without relying solely on whole-animal experiments.
An in vitro culture system reduces the complexity of whole-animal studies by allowing researchers to focus on neural stem cell responses under controlled conditions. It can therefore help identify changes in cellular behavior associated with a treatment or biological signal. Findings from the culture model provide focused evidence about cell-level processes that may later be examined in more complex settings.
Applications include investigations of neural development, cellular signaling, neurodegenerative disease, and regenerative biology. The system also supports research into approaches for repairing damaged neural tissue by allowing scientists to examine how neural stem cells respond to defined experimental conditions. These uses connect basic cell biology with questions about disease mechanisms and potential neural repair strategies.