Mouth Pipette

A mouth pipette is a laboratory device and manual technique for drawing and transferring small volumes of liquid through a glass or plastic pipette using oral suction, historically used in biology and medical laboratories. The operator creates negative pressure by inhaling through the pipette, allowing liquid to rise into the narrow tube before dispensing it into another container. Because this method can expose users to toxic, infectious, or contaminated materials and may cause accidental ingestion, modern laboratory practice prohibits mouth pipetting and requires mechanical or electronic pipette aids. Understanding the technique remains relevant for laboratory safety, historical methods, and the development of reliable liquid-handling procedures.

Mouth Pipette - Related Videos

Education

JoVE Core - Medical-Surgical Nursing

Assessment of the Mouth

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2025

A thorough mouth assessment, including inspection and palpation of the lips, gums, tongue, tonsils, uvula, and pharynx, is crucial in detecting potential health issues. Diseases ranging from oral cancer to systemic conditions like diabetes could be identified early through careful oral examination. This article provides a detailed guide on conducting a comprehensive mouth assessment. Mouth Inspection The inspection begins with visually examining the mouth for symmetry, color, and size.

Introduction to Serological Pipettes and Pipettors

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2023

The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...

Research

JoVE Journal - Biology

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

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Cited by 22 •

2008

This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

Pressure-polishing Pipettes for Improved Patch-clamp Recording

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Cited by 13 •

2008

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

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Cited by 2 •

2023

This article presents a streamlined protocol for establishing a pulpitis model in mice using an innovative mouth-gag, followed by subsequent histological analysis.

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