Ms/ms Analysis
Ms/ms Analysis - Related Videos
11:16
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HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells. Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.
10:49
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A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.
06:49
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10:22
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
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This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
FAQs
Selecting a precursor ion focuses the experiment on one mass-to-charge feature rather than the entire ion mixture. That ion is then subjected to collision-induced dissociation, producing product ions derived from it. Comparing the resulting pattern with the precursor measurement helps connect a molecular signal to structural or sequence information, which is especially useful for peptides and other biomolecules.
Collision-induced dissociation is the step that converts an isolated precursor into a set of product ions. The collection of fragments carries more interpretive information than the precursor mass alone because their pattern can reflect molecular structure and sequence. In biological analysis, this distinction supports characterization of peptides, proteins, metabolites, and molecules bearing post-translational modifications.
For peptides, product-ion patterns provide fragment-level evidence that can be related to sequence. Changes associated with post-translational modifications can also be mapped through the fragment information. This makes the analysis more informative than reporting only a molecule’s overall mass, supporting protein identification and characterization in proteomics.
A typical workflow begins by ionizing compounds, separating ions according to mass-to-charge ratio, and isolating a precursor ion. Collision-induced dissociation then generates product ions, whose measured pattern is interpreted for molecular structure or sequence. When liquid chromatography is included, it forms part of the broader analytical workflow for complex biological samples.
Liquid chromatography is often used alongside MS/MS analysis when biological samples contain complex mixtures. Its inclusion places the mass-spectrometric measurements within a broader separation-and-analysis workflow, allowing molecular signals to be examined in samples where many compounds are present. This combination is particularly relevant to proteomic and metabolomic studies of biological material.
MS/MS analysis can help investigate cellular pathways, disease-associated molecular changes, and biochemical interactions. It does so by providing evidence tied to particular peptides, proteins, metabolites, or modified molecules rather than only broad sample-level patterns. Consequently, researchers can use the measurements in proteomics and metabolomics to identify molecules and quantify or characterize them in biological studies.