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JoVE Journal
Biology

This content is Open Access.

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
 

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Article DOI: 10.3791/51553
July 12th, 2014

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Summary July 12th, 2014

HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells.  Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.

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