Multiplexed Biochip
Multiplexed Biochip - Related Videos
11:19
Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
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Cited by 12 •
2016
This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
13:14
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
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Cited by 4 •
2015
Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol describes how expression of distinct fluorescent proteins through genetic engineering is used for barcoding individual cells. The procedure enables tracking distinct populations in a cell mixture, which is ideal for multiplexed applications.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Cited by 19 •
2011
Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
FAQs
Multiplexed biochips distinguish targets by assigning different probes, antibodies, nucleic acids, or cells to separate sensing regions. Each region selectively captures its intended analyte, while optical, electrical, or chemical signals report the resulting detection event. A specialized reader then interprets the combined signals according to their spatial positions, allowing researchers to compare multiple target-specific measurements from one sample.
Integrated microfluidics controls how a biological sample moves across the sensing regions and can reduce reagent use. Controlled delivery helps expose the array to biological material in an organized way while conserving sample-associated reagents. This integration supports miniaturized analysis and is particularly useful when researchers need several measurements from a limited sample.
A multiplexed biochip measures several biological targets within one analytical run instead of focusing on one target at a time. This broader readout can reveal relationships among biological molecules that a single-target measurement may miss. It also increases the information obtained from a small sample and supports higher-throughput analysis when researchers need a coordinated molecular profile.
The platform can generate optical, electrical, or chemical signals from its individual sensing regions. Because each region is associated with a particular probe, antibody, nucleic acid, or cell, the spatial pattern of signals preserves target-specific information. A specialized reader combines and interprets these signals, producing a multi-target readout rather than a single measurement.
A typical workflow begins by delivering a biological sample through the chip, allowing different analytes to interact with designated sensing regions. The resulting signals are then collected and interpreted by a specialized reader. Integrated microfluidics may regulate sample delivery and conserve reagents. This sequence converts simultaneous target capture into a combined analytical readout for biological analysis.
Researchers can apply multiplexed biochips to gene expression analysis, protein profiling, pathogen detection, biomarker studies, and drug screening. The suitable application depends on which biological targets are placed in the sensing array. Across these areas, simultaneous measurements help generate broader molecular information, support comparisons among targets, and reveal relationships that isolated measurements may overlook.