Nanodrop Spectrophotometer
Nanodrop Spectrophotometer - Related Videos
10:29
Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
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Cited by 129 •
2009
Microvolume samples are quantified by a spectrophotometer system that uses natural surface tension to retain samples without the use of cuvettes or capillaries. The dynamic range of protein concentrations and speed by which they can be measured are greatly increased with this method.
09:28
NanoDrop Microvolume Quantitation of Nucleic Acids
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Cited by 426 •
2010
The use of NanoDrop microvolume systems as practical and efficient alternatives to traditional nucleic acid quantitation methodology is described through the demonstration of two microvolume nucleic acid quantitation protocols.
07:38
Introduction to the Spectrophotometer
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2023
The spectrophotometer is a routinely used instrument in scientific research. Spectrophotometry is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through the sample using a spectrophotometer. In this video, basic concepts in spectrophotometry, including transmittance, absorbance and the Beer-Lambert Law are reviewed in addition to the components of the spectrophotometer. These concepts provide a foundation for how to determine the...
07:44
Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
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Cited by 6 •
2010
Demonstration of quantification of dsDNA using Molecular Probes PicoGreen dye and Hitachi F-7000 Fluorescence Spectrophotometer equipped with a microplate reader accessory.
06:15
Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
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Cited by 19 •
2011
This communication presents data on the accuracy and reproducibility of the BioSpec-nano UV-VIS spectrophotometer for dsDNA and protein quantitation. Even with ultra-small volumes (1 to 2 L), reproducibility is excellent, while the automated wiping function improves throughput and results in minimal carryover for more precise results.
FAQs
The instrument applies the Beer–Lambert law to light absorbed by the sample. A small liquid column forms between two optical surfaces, and the measured absorbance at selected wavelengths is used to estimate the amount of DNA, RNA, or protein present. This links an optical measurement to concentration without requiring a large sample volume.
Different measurement wavelengths provide information used to assess both sample amount and quality. Absorbance readings support concentration estimates, while ratios between readings help identify contamination. In practice, researchers interpret these values together rather than relying on concentration alone, because a sample with sufficient biological material may still be unsuitable for downstream analysis if contaminants affect its purity.
The liquid column bridges the instrument’s optical surfaces, creating the measurement path through which light passes. Because the sample itself forms this path, only a small volume is needed for absorbance analysis. This design supports rapid testing of biological samples while conserving DNA, RNA, proteins, or other limited material used in laboratory workflows.
Concentration indicates how much biological material the sample contains, whereas purity measurements provide evidence about potential contaminants. Considering both results helps determine whether DNA, RNA, or protein is appropriate for a planned experiment. This combined assessment is especially useful before procedures where sample quality can affect the reliability of downstream biological analysis.
A basic workflow involves placing a small liquid sample between the instrument’s optical surfaces, selecting the relevant measurement wavelengths, and recording absorbance-based concentration and purity results. Researchers then evaluate those results against the needs of the intended experiment. The procedure provides rapid sample quality control while using minimal biological material.
Researchers use the instrument during sample quality control before applications such as PCR, sequencing, cloning, or protein analysis. The measured concentration helps assess available material, while purity information can reveal whether contaminants may interfere with subsequent work. Its low-volume, rapid workflow is also useful in biology research and teaching laboratories.