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Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
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Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

DOI:
10.3791/1610-v

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10:29 min

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November 04, 2009

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, ,
1Thermo Scientific NanoDrop Products

Chapters

  • 00:00Title
  • 00:39Introduction
  • 00:59The NanoDrop 2000c Spectrophotometer
  • 02:27Determining Microvolume Protein Concentration Using A280 Absorbance Measurements
  • 05:47Determining Microvolume Protein Concentration Using Colorimetric Assays
  • 10:03Conclusion

Summary

Automatic Translation

Automatic Translation

Microvolume samples are quantified by a spectrophotometer system that uses natural surface tension to retain samples without the use of cuvettes or capillaries. The dynamic range of protein concentrations and speed by which they can be measured are greatly increased with this method.

Tags

MicrovolumeProtein Concentration DeterminationNanoDrop 2000c SpectrophotometerTraditional SpectrophotometrySample Retention SystemMeasurement SurfacesSurface Tension PropertiesReal Time Changes In Path LengthDynamic RangeProtein ConcentrationsDilutionsSample QuantificationA280 Absorbance ReadingsBCA Colorimetric Assay

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