Ng2 Surface Marker
Ng2 Surface Marker - Related Videos
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
09:38
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
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Cited by 38 •
2011
Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Cited by 1 •
2019
Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
FAQs
NG2’s molecular organization affects how it is detected: a transmembrane core protein presents extracellular regions, and attached glycosaminoglycan chains contribute to its proteoglycan structure. Antibodies recognize exposed portions of the molecule rather than the intracellular region. This architecture allows investigators to translate surface expression into a measurable signal while preserving the distinction between a cell-surface marker and an internal protein.
NG2 expression is especially informative when studying oligodendrocyte precursor cells because it can be followed before and during differentiation. Comparing NG2-positive cells with their differentiation state helps researchers examine how precursor populations change as they develop. In nervous-tissue studies, this makes the marker useful for organizing analyses of glial development and myelin repair without treating marker detection as the entire biological outcome.
Interpretation depends on the tissue being examined. NG2-positive populations are associated not only with oligodendrocyte precursor cells but also with vascular pericytes, so a positive signal may represent different biological populations in nervous and vascular contexts. Researchers therefore use the marker within a defined tissue and experimental question, rather than assuming that every labeled cell has the same identity or function.
The appropriate detection method depends on whether the goal is spatial localization, population measurement, or physical separation. Immunostaining shows where antibody-bound signal appears in tissue, flow cytometry measures labeled cells as a population, and cell sorting uses the signal to separate selected cells. These approaches can address complementary questions about distribution, abundance, and downstream analysis.
An NG2 antibody provides the recognition step in each assay by binding an exposed region of CSPG4. That binding makes the surface marker visible to an immunostaining workflow or measurable for flow cytometry and sorting. The antibody therefore connects molecular presence with an experimental readout, while the chosen platform determines whether researchers observe location, quantify a population, or collect cells.
Tracking NG2-positive cells can help investigators examine how progenitor or tumor-associated populations change in disease and after tissue injury. In nervous-system research, the same strategy supports studies of glial development and myelin repair; in vascular tissue, it can support analysis of pericyte-associated populations. The value lies in comparing labeled populations across biological contexts and experimental conditions.