Non-invasive Imaging
Non-invasive Imaging - Related Videos
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
01:37
Non-invasive Monitoring and Imaging in Animal Research
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Non-invasive technologies support the principles of 3Rs by enabling repeated data collection from the same animal without the need for euthanasia at multiple time points. These approaches reduce the number of animals required...
07:08
Modeling and Imaging 3-Dimensional Collective Cell Invasion
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Cited by 20 •
2011
Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.
07:42
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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Cited by 33 •
2008
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
FAQs
Image formation begins with signal capture: a method records transmitted light, emitted fluorescence, reflected ultrasound, or magnetic responses from the living system. Computational reconstruction then converts those measurements into a spatial representation of structures or activity. The resulting image is therefore an interpreted signal pattern, and its biological meaning depends on which signal the technique detects.
Different approaches provide complementary views because they rely on different signal sources. Transmitted light can reveal patterns based on passage through tissue, fluorescence can indicate emitted signals, ultrasound uses reflected ultrasound, and magnetic methods detect magnetic responses. Comparing these options helps align the imaging approach with the biological feature or activity a study aims to observe.
Avoiding surgical removal of tissue reduces disruption to the organism being observed. This matters when researchers want measurements that reflect anatomy, physiology, development, disease progression, or treatment response under preserved conditions. The approach can therefore support observations that would be harder to interpret if the imaging procedure itself substantially altered the biological system.
Repeated imaging of the same organism creates a time-resolved record rather than a single snapshot. Researchers can compare anatomy, physiology, development, disease progression, or treatment response across observation points while retaining organism-specific context. This longitudinal design strengthens analysis of how biological systems change over time and reduces reliance on comparisons between separate organisms.
A general workflow starts by selecting a detectable biological signal, collecting measurements from the living system, and applying computational reconstruction to produce an image. Researchers can then examine the image for anatomical or functional patterns and repeat the process at later time points. This sequence links raw signal detection with biological interpretation without requiring tissue removal.
Non-invasive Imaging can be applied to questions about anatomy, physiology, development, disease progression, and treatment response. The same general capability supports both structural observations and measurements of activity, allowing a study to follow how a biological system changes over time. Its value is greatest when preserving the living system is important to the research question.
At the cellular and molecular end, imaging can support investigations of biological structures or activity; at the whole-organism level, it can follow anatomy, physiology, development, disease, or treatment response. This range lets researchers examine biology across scales while retaining the possibility of repeated observation, linking detailed investigations with changes occurring throughout a living system.