Olfactory Epithelium

The olfactory epithelium is a specialized sensory tissue in the nasal cavity that detects airborne chemicals and initiates the sense of smell. Its olfactory receptor neurons extend cilia into the mucus layer, where odorant molecules bind receptor proteins and trigger signaling that converts chemical stimuli into electrical impulses; supporting cells and basal stem cells help maintain the tissue. Neuronal axons transmit these signals to the olfactory bulb, where odor information is organized and interpreted by the brain. Studying this epithelium supports research on sensory biology, neural regeneration, environmental odor detection, and disorders that impair smell.

Olfactory Epithelium - Related Videos

Research

JoVE Journal - Neuroscience

Rapid Dissection and Dissociation of the Mouse Olfactory Epithelium for Single-Nucleus Suspensions

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Cited by 1 •

2025

This article provides a streamlined protocol for the dissection and dissociation of murine olfactory epithelium for the purpose of single-nucleus RNA sequencing.

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

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Cited by 13 •

2014

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

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2017

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Research

JoVE Journal - Neuroscience
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Isolating Nasal Olfactory Stem Cells from Rodents or Humans

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Cited by 98 •

2011

We describe here a method for biopsying olfactory mucosa from rat and human nasal cavities. These biopsies can be used for either identifying molecular anomalies in brain diseases or isolating multipotent adult stem cells that can be utilized for cell transplantation in animal models of brain trauma/disease.

Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice

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2025

This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...

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