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Isolating Nasal Olfactory Stem Cells from Rodents or Humans
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Neuroscience
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JoVE Journal Neuroscience
Isolating Nasal Olfactory Stem Cells from Rodents or Humans

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

DOI:
10.3791/2762-v

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09:19 min

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August 22, 2011

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, , , , ,
1NICN,Aix Marseille University, 2LNPM,Aix Marseille University, 3ENT Department,Aix Marseille University, 4Gene expression Laboratory,The Salk Institute for Biological Studies, 5Laboratory of Speech and Language,Aix Marseille University, 6Centre d’Investigations Cliniques en Biothérapie,Aix Marseille University

Chapters

  • 00:05Title
  • 02:08Collection of Olfactory Mucosa in Rats
  • 03:26Collection of Olfactory Mucosa in Humans
  • 04:48Isolation of Olfactory Stem Cells from Human and Rat Mucosa
  • 06:47Sphere Formation and Neuronal Differentiation of Olfactory Stem Cells
  • 07:33Human Olfactory Stem Cell Characteristics
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a method for biopsying olfactory mucosa from rat and human nasal cavities. These biopsies can be used for either identifying molecular anomalies in brain diseases or isolating multipotent adult stem cells that can be utilized for cell transplantation in animal models of brain trauma/disease.

Tags

Nasal Olfactory Stem CellsRodentsHumansOlfactory MucosaExternal EnvironmentMolecular AnomaliesPathological BrainAdult Stem CellsCell TherapyOlfactory EpitheliumNeurogenesisComparative Molecular StudiesIn Vitro ExperimentsNeuroepitheliumLamina Propria

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