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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

DOI:
10.3791/55893-v

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06:32 min

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June 05, 2017

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, ,
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Chapters

  • 00:05Title
  • 01:11Preparation of Parasagittal Olfactory Bulb Sections
  • 02:33Day 1: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:07Day 2: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:56The Use of Fluorescence Intensity Measurements
  • 04:26Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Tags

Keywords: Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

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