Ovarian Cortex Cryopreservation
Ovarian Cortex Cryopreservation - Related Videos
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 2 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
06:32
Bovine Ovarian Cortex Tissue Culture
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Cited by 7 •
2021
In vitro culture of bovine ovarian cortex and the effect of nutritional Stair-step diet on ovarian microenvironment is presented. Ovarian cortex pieces were cultured for seven days and steroids, cytokines, and follicle stages were evaluated. The Stair-Step diet treatment had increased steroidogenesis resulting in follicle progression in culture.
05:53
Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation
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2024
A protocol for the vitrification of ovarian tissue, as an alternative cryopreservation method to the widely used slow freezing protocol, is presented.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
FAQs
The ovarian cortex is valuable because it contains many primordial follicles, which are the follicle population being preserved. Cryoprotective agents and either controlled slow cooling or vitrification reduce ice-crystal formation and associated cellular injury. Maintaining this tissue-level viability creates the basis for evaluating whether follicles can later retain survival and function.
Cryoprotective agents help limit cellular injury during exposure to very low temperatures, while the cooling method influences how effectively ice-crystal formation is controlled. Ovarian cortex cryopreservation may use controlled slow cooling or vitrification. These approaches provide different ways to reduce freezing-related damage and support tissue viability during later warming.
Warming can reveal whether follicles and surrounding ovarian tissue remained viable after storage. Researchers assess both survival and function because preserved tissue must retain more than structural integrity to support possible future use. These evaluations also provide biological information about how cryopreservation affects follicle maintenance and activity.
The process includes exposure of ovarian cortical tissue to cryoprotective agents, cooling by controlled slow cooling or vitrification, storage at very low temperatures, and subsequent warming. After warming, investigators assess follicle survival and function. This sequence connects the preservation conditions with measurable tissue outcomes and helps determine whether viability was maintained.
This approach may be relevant when cancer treatment could damage ovarian function, when ovarian surgery may reduce ovarian reserve, or when another condition threatens reproductive potential. Its value lies in preserving tissue before the anticipated threat. The stored cortex can then be evaluated for possible future reproductive or endocrine use.
In biology research, preserved ovarian cortex provides a system for examining follicle activation and tissue viability after warming. Studies can also investigate whether ovarian tissue contributes to restoration of endocrine and reproductive activity. These applications connect cryopreservation with broader questions about follicle biology, ovarian function, and tissue recovery.