Ovarian Structure
Ovarian Structure - Related Videos
13:04
In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
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Cited by 10 •
2015
We outline a protocol that implements both in vivo and ex vivo approaches to study ovarian cancer colonization of peritoneal adipose tissues, particularly the omentum. Furthermore, we present a protocol to quantitate and analyze immune cell-structures in the omentum known as milky spots, which promote metastases of peritoneal adipose.
04:35
Obtaining Primary Ovarian Cancer Cells from Solid Specimens: A Method to Culture Epithelial Ovarian Cancer Cells from Ovarian Tumor Specimens
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2023
In this video, we describe a method for isolating and characterizing primary ovarian cancer cells from solid ovarian tumor tissue specimens. The epithelial cancer cells obtained using this protocol are suitable for understanding the processes that may lead to ovarian cancer initiation and development.
10:35
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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Cited by 18 •
2010
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
FAQs
The cortex contains follicles at different developmental stages, creating a structural record of progression toward oocyte maturation. Comparing these stages helps researchers relate ovarian organization to follicular growth, hormonal regulation, and the possibility of ovulation. Changes in the number or appearance of follicles can therefore provide context for studying fertility and ovarian aging.
The medulla provides an internal support region containing connective tissue, blood vessels, lymphatic vessels, and nerves. These components help sustain the surrounding ovarian tissue and support its functional activity. Considering the medulla alongside the follicle-containing cortex is important when interpreting how ovarian structure maintains reproductive and hormone-related processes.
Hormonal regulation directs follicle growth and helps coordinate the transition toward ovulation. After a mature oocyte is released, the remaining follicular tissue may form a corpus luteum, which secretes hormones. This structural transition links changes in ovarian tissue to endocrine control of the menstrual cycle and fertility.
A developing follicle represents tissue progressing toward release of a mature oocyte, whereas a corpus luteum forms from follicular tissue after ovulation. Their different stages reflect distinct ovarian activities: follicular development supports oocyte maturation, while the corpus luteum contributes hormone secretion. This distinction helps connect ovarian anatomy with cycle timing.
Examining ovarian structure across the cycle can reveal how follicles progress, when one may release a mature oocyte, and how residual tissue changes afterward. These observations connect physical organization with hormonal activity and reproductive timing. They also provide a framework for investigating disruptions associated with fertility problems, ovarian aging, cysts, or tumors.
Structural organization gives researchers a way to relate ovarian function to disease and reproductive outcomes. Follicular changes can be considered in studies of fertility and ovarian aging, while abnormalities involving ovarian tissue are relevant to cyst and tumor research. The same framework also supports investigation of endocrine regulation and menstrual-cycle disturbances.