Ovariectomized Mice
Ovariectomized Mice - Related Videos
08:56
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
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Cited by 7 •
2023
Presented here is a protocol to show that Bazi Bushen capsule (BZBS) can regulate the RANKL/OPG signaling pathway in the ovariectomized rodent model through its estrogen-like effect.
06:49
Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model
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2023
Pregnancy establishment is a dynamic process involving complex embryo and uterine crosstalk. The precise contributions of the maternal uterine environment to these processes remain an active area of investigation. Here, detailed protocols are provided to aid in designing in vivo animal models to address these research questions.
06:05
Anticipatory Food-Reward Elicitation of Appetitive Ultrasonic Vocalizations in Laboratory Rats and Mice
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2026
Laboratory rat and mouse ultrasonic vocalizations (USVs) are useful for studying vocal and neural function. This protocol describes a food-reward paradigm that reliably elicits appetitive USVs without mating-related confounds. It details animal conditioning, stimulus presentation, recording, and acoustic analysis, providing a reproducible method for studying affective-motivational and cranial sensorimotor function.
06:17
Measuring the Strength of Mice
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Cited by 287 •
2013
Deficits in muscular strength occur in many clinical conditions such as motor neuron disease. The inverted screen and weight lifting tests described here measure strength in mice almost exclusively, with minimal influence of factors such as coordination.
36:30
Small Bowel Transplantation In Mice
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Cited by 2 •
2007
The mouse small bowel transplantation model has been recognized as an important tool to study mechanismes of immune rejection and screen new immunosuppressive drugs. However, this model is limited to use because the techniques involved is an extremely technically challenge. Now we introduce the modified technique.
FAQs
The key experimental change is the reduction of circulating estrogen and progesterone after ovarian removal. This depletion creates a biological contrast between hormone-intact and hormone-depleted states, allowing investigators to examine physiological changes associated with loss of ovarian hormonal input. The model therefore links observed tissue responses to altered endocrine conditions rather than ovarian function alone.
Estrogen and progesterone serve as the principal hormonal variables in this model. Their reduced circulation provides a basis for asking how ovarian hormones regulate particular tissues and biological functions. By examining responses after depletion, researchers can separate hormone-dependent effects from broader disease or treatment effects, especially when matched controls are included.
Sham-operated and untreated mice provide different reference points for evaluating physiological changes. Comparing these groups with ovariectomized mice helps distinguish effects associated with ovarian hormone depletion from differences present without the experimental intervention. This design strengthens interpretation when researchers assess tissue responses, disease-related changes, or treatment outcomes.
Age, strain, and recovery time can influence how results are interpreted in ovariectomized mice. These variables should be considered when comparing physiological changes or treatment responses, because differences between studies may reflect model context as well as hormone depletion. Explicitly accounting for them improves comparisons with sham-operated or untreated controls.
These mice support investigations of menopause-related bone loss, reproductive endocrinology, metabolism, cardiovascular function, and hormone-responsive disease. Their value lies in applying one controlled endocrine manipulation across diverse biological outcomes. Researchers can therefore examine whether ovarian hormone depletion is associated with tissue-specific changes and compare those outcomes with appropriate control groups.
Researchers can compare ovariectomized mice receiving hormone replacement or another intervention with relevant control groups to assess therapeutic outcomes. The model makes it possible to test whether treatment responses emerge under reduced estrogen and progesterone conditions. Such comparisons can connect a proposed therapy with changes in hormone-responsive tissues or functions studied in the experiment.