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Chapters
- 00:03Title
- 00:16Introduction
- 01:18Harvesting Small Bowel from Donor Mouse
- 01:201. performing Tail Pinch to Ensure Mouse is Anesthetized
- 02:462. clearing away tissue to allow ligation of small bowel
- 04:213. cleaning out small bowel contents before ligating ileum
- 04:504. ligating ileum
- 05:205. exposing and ligating mesenteric vessels
- 06:186. exposing mesenteric artery which will be cut when small bowel is
- 06:427. exposing portal vein
- 07:288. ligating branch of portal vein
- 07:589. exposing other side of mesenteric artery
- 08:0910. locating inferior vena cava
- 08:1511. injecting heparin into the inferior vena cava
- 08:3612. cutting mesenteric artery
- 08:5413.clearing away tissue from mesentaric artery
- 09:2314. cutting jejunal end of ligated small bowel
- 09:3715. cutting away small bowel mesenteric tissue
- 10:2716. cutting away mesenteric tissue until the portal vein is reached
- 10:5617. cutting ileal end of ligated small bowel
- 11:1918. cutting away mesenteric tissue
- 11:3419. cutting portal vein
- 11:5720. harvesting donor small bowel
- 12:09Preparing Recipient Mouse for Small Bowel Transplantation
- 13:201. exposing inferior vena cava and aorta
- 13:292. clearing away tissue in order to apply ligatures
- 14:353. applying ligatures to inferior vena cava and inferior aorta
- 15:03Connecting Donor Small Bowel Circulation to Recipient Circulation
- 15:151. placing donor small bowel in recipient mouse
- 15:56puncturing inferior vena cava for veinotomy
- 16:173. performing veinotomy on inferior vena cava
- 16:314. repeat procedure for aorta
- 17:005. washing away blood from vessels with saline
- 17:156. suturing donor mesenteric artery to recipient aorta
- 19:597. suturing donor portal vein to recipient inferior vena cava
- 22:398. final suture for anastamosis of donor portal vein to recipient i
- 23:019. flipping donor small bowel in order to finish suture of mesenter
- 23:3510. suturing donor mesenteric artery to other side of aorta
- 24:5311. final suture for anastamosis of donor mesenteric artery to reci
- 25:5612. releasing ligatures to open circulation to donor small bowel
- 26:47Connecting Donor Small Bowel to Recipient Small Bowel
- 27:201. clamping ileal end of donor small bowel
- 27:392. cutting recipient jejunum
- 27:503. cutting ileal end of donor small bowel
- 28:044. suturing one side of donor ileum to recipient jejunum
- 29:105. flipping small bowel clamps in order to suture other side of ana
- 30:206. removing clamps
- 31:147. creating stoma for jejunal end of donor small bowel
- 32:028. suturing jejunal end of donor small bowel to stoma
- 32:409. cutting ligature to jejunal end of donor small bowel
- 33:14Sewing up Abdominal Cavity
- 35:32Conclusion
The mouse small bowel transplantation model has been recognized as an important tool to study mechanismes of immune rejection and screen new immunosuppressive drugs. However, this model is limited to use because the techniques involved is an extremely technically challenge. Now we introduce the modified technique.
Tags
Small Bowel TransplantationMiceTacrolimus-based ImmunosuppressionSurgical TechniquesImmunosuppressive MedicationsInfection ProphylaxisPatient SelectionGraft Survival RatesPatient Survival RatesIrreversible Intestinal FailureComplications Of Parenteral NutritionRenal TransplantationLiver TransplantationHeart TransplantationLung TransplantationImmunology Of Transplantation GutPhysiology Of Transplantation GutAnimal ModelsMouse Small Bowel Transplantation ModelTechniques Of Small Bowel Transplantation
