Ovary Dissection

Ovary dissection is a laboratory technique used to expose and examine the internal anatomy of an ovary, making reproductive structures accessible for biological study. During the procedure, the specimen is positioned and cut with fine instruments, allowing the outer cortex and inner medulla to be distinguished and follicles, oocytes, or other visible features to be observed; preserved tissues may also be prepared for microscopy. In biology education and research, this method links gross anatomy with ovarian function, follicle development, and reproductive cycles. It supports comparative anatomy, developmental studies, and investigation of how ovarian structure changes across life stages or experimental conditions.

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JoVE Journal - Biology

Dissection and Staining of Drosophila Larval Ovaries

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Cited by 28 •

2011

How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development. This video demonstrates how to dissect, stain and mount female gonads from late third instar (LL3) Drosophila larvae.

Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation

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2023

This video describes the general anatomy of the Drosophila ovary and features a method of dissecting ovaries for live or fixed imaging.

Dissection of Drosophila Ovaries

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Cited by 28 •

2006

Dissection and Staining of Drosophila Pupal Ovaries

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Cited by 4 •

2018

The Drosophila ovary is an excellent model system for studying stem cell niche development. Though methods for dissecting larval and adult ovaries have been published, pupal ovary dissections require different techniques that have not been published in detail. Here we outline a protocol for dissecting, staining, and mounting pupal ovaries.

Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model

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2025

In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.

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