Paris Japonica

Paris japonica is a flowering plant in the family Melanthiaceae and has the largest known nuclear genome among plants, making it an important subject for studying genome size and evolution. Its genome contains roughly 150 billion base pairs, an enormous size produced largely by polyploidy and the accumulation of repetitive DNA, including transposable-element sequences, combined with limited DNA removal. Research on Paris japonica helps scientists investigate how genome expansion affects chromosome organization, cell division, development, and ecological adaptation. It also provides a valuable comparison for understanding genome evolution across flowering plants and the biological trade-offs associated with very large genomes.

Paris Japonica - Related Videos

Education

JoVE Core - Molecular Biology

Genome Size and the Evolution of New Genes

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2020

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.

Research

JoVE Journal - Biology
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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica

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Cited by 17 •

2011

An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

Protocols for Efficient Rearing and Functional Analysis of the Endoparasitoid Wasp Asobara japonica and its Host Drosophila melanogaster

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2026

The goal of this protocol is to facilitate functional analysis of parasitoid-host interactions in Asobara japonica and Drosophila melanogaster through optimized rearing methods, a single-oviposition infection assay, and RNAi-mediated gene knockdown.

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

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Cited by 5 •

2016

MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

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Cited by 12 •

2019

Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also provided.

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