Passive Crosslinkers
Passive Crosslinkers - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
10:34
Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
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Cited by 6 •
2016
A protocol about the characterization and application of five different passive sampling devices is presented.
01:27
Passive Filters
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2024
Passive filters are utilized to shape the frequency spectrum of signals across a diverse array of applications. These filters, using only passive elements like resistors (R), inductors (L), and capacitors (C), are capable of selectively allowing or blocking certain frequency ranges without the need for external power sources. Low-Pass Filters Low-pass filters are designed to transmit signals with frequencies lower than the cutoff frequency, ωc, and attenuate those above it. The cutoff frequency...
FAQs
Covalent crosslinking creates strong chemical connections between neighboring macromolecules, whereas reversible interactions can provide a network that is not permanently fixed. These two mechanisms influence how the resulting material maintains cohesion, stiffness, and resistance to dissolution. The distinction is important when designing biomaterials because network stability must be balanced with properties such as degradation and compatibility with living systems.
Crosslinker concentration helps determine how extensively polymer chains become connected. Changing it can alter the material’s stiffness, porosity, resistance to dissolution, and degradation behavior. Because these properties also affect biological compatibility, concentration is a central design variable for hydrogels and extracellular-matrix-like scaffolds. Researchers can therefore adjust the network to suit cell culture, tissue engineering, or delivery applications.
Passive crosslinkers maintain the network without requiring ongoing energy input or active environmental control. This distinguishes them from approaches that depend on continued stimulation or regulation after formation. The resulting stability can simplify the use of crosslinked materials in biological settings, where hydrogels, scaffolds, and encapsulation systems must retain their structure without continuous intervention.
Selection requires balancing network cohesion with biological compatibility. A suitable choice should provide enough stiffness and resistance to dissolution for the intended material, while preserving useful porosity and an appropriate degradation profile. The chemical or physical interaction used, together with its concentration, influences these outcomes and helps determine whether the network is appropriate for cells, tissue-engineering constructs, or biomolecular cargo.
Design begins by choosing the crosslinking interaction and then considering its concentration in relation to the polymer network’s intended function. Those choices shape cohesion, stiffness, porosity, degradation, and compatibility. The resulting network can be configured as a hydrogel or an extracellular-matrix-like scaffold, allowing the material’s mechanical and dissolution-related behavior to match a biological application.
They are useful when researchers need materials that combine structural cohesion with tunable mechanical properties. Applications described for these networks include cell culture, tissue engineering, drug delivery, and biomolecular encapsulation. Their value comes from the ability to adjust network characteristics such as stiffness, porosity, degradation, and resistance to dissolution through the crosslinker choice and concentration.
In biology, crosslinked networks can stabilize scaffolds that mimic aspects of the extracellular matrix while providing controlled mechanical properties. Their stiffness and porosity influence the physical environment available within the material, and their degradation and compatibility affect suitability for living systems. This makes them relevant to cell culture and tissue engineering, where both structural support and biological tolerability are important.