Primitive Streak
Primitive Streak - Related Videos
14:37
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Cited by 19 •
2017
Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.
09:03
Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
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2023
Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...
10:17
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
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Cited by 3 •
2021
The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.
07:24
Development of the Chick
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2023
The chicken embryo (Gallus gallus domesticus) provides an economical and accessible model for developmental biology research. Chicks develop rapidly and are amenable to genetic and physiological manipulations, allowing researchers to investigate developmental pathways down to the cell and molecular levels. This video review of chick development begins by describing the process of egg fertilization and formation within the chicken reproductive tract. Next, the most commonly used chick staging...
09:57
Dissection of 6.5 dpc Mouse Embryos
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Cited by 28 •
2007
Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).
FAQs
Epithelial-to-mesenchymal transition enables epiblast cells to change their behavior as they approach the streak. This transition is closely associated with their ingress between existing cells, allowing the cells to leave the original layer and participate in forming mesoderm and definitive endoderm. It therefore links cellular behavior with the tissue reorganization required during early development.
Epiblast cells first move toward the primitive streak and then pass between existing cells through ingress. These coordinated movements redistribute cells within the embryo rather than simply adding new cells to its surface. Their positions after movement help generate distinct germ-layer populations, making migration an essential mechanism for both tissue organization and lineage formation.
Primitive streak formation is important because the associated cellular movements help establish the embryo’s body axes. As cells converge on, enter, and move through the streak, their changing positions contribute to the spatial organization of the developing embryo. Studying this relationship helps connect cell movement with the emergence of an ordered body plan.
Cells entering through the primitive streak contribute to mesoderm and definitive endoderm, while the surrounding epiblast provides the developmental context for these changes. This makes the streak a point where physical movement and lineage specification occur together. Its study helps explain how early embryonic cells become organized into populations with different developmental contributions.
Embryonic and induced pluripotent stem-cell systems can be used to model developmental events associated with primitive streak formation. Such models provide a way to investigate how early cell movements, tissue patterning, and lineage specification are coordinated. They extend study beyond the embryo itself and support controlled examination of processes involved in early development.
Research on the primitive streak can clarify how early developmental processes become organized and how disruptions may relate to congenital disorders. Stem-cell-based models also use this developmental context to investigate differentiation and produce specialized cell types. Together, these applications connect basic embryology with disease research and efforts to guide cells toward defined developmental outcomes.