Protein Delivery Method
Protein Delivery Method - Related Videos
12:54
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
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Cited by 26 •
2014
In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.
01:26
Methods Of Healthcare Delivery System
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2023
At the different levels of the healthcare system, we see varying methods of healthcare used. These methods include managed care systems, case management, and primary healthcare. Managed Care System: The managed care system is designed to control the cost while maintaining the quality of care. The patient's care from admission to discharge is planned by the primary care provider or the case manager, also known as the gatekeeper. In a managed care system, the number of care providers is limited...
02:20
Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells
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2025
Source: Lee, H. et. al., Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection. J. Vis. Exp. (2018)This video demonstrates a method for monitoring bacterial infection and effector protein delivery in plant cells using split superfolder green fluorescent protein (sfGFP) complementation combined with confocal microscopy. The approach enables precise visualization of effector translocation into the host cell cytosol.
18:57
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Cited by 58 •
2013
Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.
10:30
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Cited by 10 •
2015
We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.
FAQs
Many systems are designed to improve cellular uptake and support release from endosomes, membrane-bound compartments that can otherwise sequester incoming material. Encapsulation in nanoparticles, attachment to carrier molecules, or temporary membrane permeabilization can address different barriers to intracellular access. Successful delivery therefore depends not only on getting protein into a cell, but also on making it available where its activity is needed.
Direct protein delivery can provide activity without requiring gene expression, making the intervention more time-limited and directly tied to the administered protein. This distinction is valuable when researchers need to study an immediate cellular response or alter a pathway for a defined period. It also supports evaluation of protein-based therapeutic candidates without first establishing expression from introduced genetic material.
These components address different stages of the delivery challenge. Nanoparticles can encapsulate proteins, carrier molecules can link proteins to a transport strategy, and temporary membrane permeabilization can facilitate passage across the cell boundary. The selected design influences how the protein enters the biological system and whether the system can support subsequent intracellular release.
The outcome depends on more than the presence of protein in the surrounding system. Researchers must consider how the protein is transported, whether cells take it up, and whether it is released from endosomes rather than remaining trapped. These features determine how much functional protein reaches the relevant cellular environment and can affect interpretation of pathway changes or other responses.
A study generally selects a delivery design suited to the protein and biological system, introduces the protein through encapsulation, a carrier linkage, or temporary membrane permeabilization, and then examines the resulting cellular or tissue response. Evaluation can focus on whether uptake and intracellular release occur and whether the delivered protein produces the expected change in protein function or pathway activity.
These methods are useful when investigators need to examine protein function, modify cellular pathways, or test a therapeutic candidate without depending on gene expression. Applications described for biology include enzyme replacement research, vaccine design, and studies requiring precise, time-limited control of protein activity. The same flexibility makes delivery systems relevant during early assessment of protein-based interventions.