Journal
/
Bioengineering
/
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

DOI:
10.3791/50638-v

•

18:57 min

•

October 17, 2013

•

,
1Fischell Department of Bioengineering,University of Maryland, 2Institute for Bioscience and Biotechnology Research,University of Maryland

Chapters

  • 00:05Title
  • 03:09Culturing a Cell Monolayer in Transwell Inserts
  • 04:34Validation of the GI Epithelial Permeability Barrier using Transepithelial Electrical Resistance (TEER) and Immunostaining of Tight Junctions
  • 06:29Evaluating Transepithelial Transport of Targeted Carriers
  • 10:25Investigating the Mechanism of Transepithelial Transport of Targeted Nanocarriers
  • 12:36Results: Caco-2 Cell Monolayer is a Model of Transepithelial Transport of Drug Nanocarriers (NCs) Across Cellular Barriers
  • 17:50Conclusion

Summary

Automatic Translation

Automatic Translation

Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.

Tags

Keywords: Drug Delivery SystemsNanocarriersCellular BarriersParacellular TransportTranscellular TransportTransepithelial Electrical ResistanceTranswellEndocytosisTranscytosisRadioisotope Tracing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50638?article_type=v

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model
check_url/50638?article_type=v

Self-reporting Scaffolds for 3-Dimensional Cell Culture
check_url/50638?article_type=v

Manufacture and Drug Delivery Applications of Silk Nanoparticles
check_url/50638?article_type=v

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
check_url/50638?article_type=v

Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology
check_url/50638?article_type=v

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
check_url/50638?article_type=v

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods
check_url/50638?article_type=v

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
check_url/50638?article_type=v

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
check_url/50638?article_type=v

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Read Article