Radish Microgreens
Radish Microgreens - Related Videos
09:02
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This protocol describes how to construct and operate a small, low-cost growth chamber capable of maintaining extreme carbon dioxide (exCO2) levels for plant experiments. The chamber enables reproducible studies of microgreen physiology under spaceflight-relevant atmospheric conditions, using a single-board computer-based monitoring and control system and a passive hydroponic wicking system.
01:55
Energy Dynamics - Instructor Prep
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Cabbage Pre-Weighing ExpandTo obtain the percent biomass of a representative cabbage before the experiment, use a paper towel to wash and pat dry the inner leaves of a grocery store cabbage. Place the cabbage into a pre-weighed Petri dish on a balance to determine the wet mass of the vegetable, and then transfer the cabbage and the dish into a 37 °C incubator for 48 hours. At the end of the incubation, weigh cabbage in the dish again to obtain the dry mass of the cabbage. Then, calculate the...
08:51
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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Cited by 11 •
2015
The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
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Cited by 6 •
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We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
16:01
Immunoblot Analysis
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Cited by 21 •
2008
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.
FAQs
Water uptake is the first key transition: it initiates germination and enables the seed to use stored reserves for early root and shoot growth. This means initial development can proceed before nutrient availability is varied as an experimental condition. In biology investigations, researchers can therefore examine how germination progresses into visible seedling growth.
Light exposure activates chlorophyll production and photosynthesis after the cotyledons emerge. Chlorophyll gives developing tissues the capacity to capture light for photosynthetic activity, linking illumination with continued seedling growth. In biology investigations, changing light exposure can help reveal photomorphogenesis, the way light influences plant form and development, rather than merely measuring final size.
Nutrient availability, temperature, and moisture are major controllable variables because each can alter biomass, the amount of plant material produced, and morphology, the plant’s observable form. Holding two factors steady while changing one helps researchers associate differences in growth with a particular condition. This approach supports controlled studies of development and stress responses.
Their rapid growth and short production cycle make developmental changes observable within a practical experimental window. A single system can connect germination, root and shoot expansion, light responses, and stress-related changes. This makes them useful in biology teaching and controlled studies where researchers need to compare conditions and observe outcomes quickly.
A simple workflow begins by providing water to initiate germination, allowing root and shoot growth from stored reserves, and then exposing emerging seedlings to light so chlorophyll production and photosynthesis can begin. Researchers harvest after cotyledon emergence when investigating early development or producing edible material, while controlling moisture, temperature, and nutrient availability.
Measurements can focus on germination progression, root and shoot growth, biomass, morphology, chlorophyll production, or photosynthetic activity. The selected outcome should match the question: growth measures describe development, form reveals morphological responses, and chlorophyll or photosynthesis indicates a response to light. These observations allow conditions to be compared within a controlled cultivation system.
Their short production cycle supports both controlled biological experiments and practical exploration of plant-based food production. Researchers and educators can study functional food composition while examining how cultivation conditions influence growth and form. The same rapid system also provides context for sustainable food production, linking early plant biology with the development of edible plant materials.