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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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Immunology and Infection
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JoVE Journal Immunology and Infection
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

DOI:
10.3791/52903-v

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08:51 min

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June 25, 2015

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, , ,
1Department Biologie,Friedrich-Alexander-Universität, 2Institut für Molekulare Pathogenese,Friedrich-Loeffler-Institut, 3Mikrobiologisches Institut – Klinische Mikrobiologie, Immunologie und Hygiene,Universitätsklinikum Erlangen

Chapters

  • 00:05Title
  • 01:56Transfection of Stable Cell Line with Inducible Expression Vector System
  • 03:08Analysis of Host Cell Apoptosis by Immunoblot Analysis
  • 06:41Results: Effects of the Expression of GFP-CaeB on Staurosporine-induced Apoptosis
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.

Tags

Keywords: Inducible Expression SystemBacterial Virulence FactorsIntracellular SignalingApoptotic CascadeCoxiella BurnetiiCaeB Effector ProteinBaxCaspase 3Cell DeathSignaling PathwayProtein Interaction

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