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JoVE Journal
Immunology and Infection

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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
 

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Article doi: 10.3791/52903
June 25th, 2015

Summary June 25th, 2015

The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.

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