Rcf
Rcf - Related Videos
07:52
An Introduction to the Centrifuge
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2023
The centrifuge is an instrument used in nearly every research lab across the globe. Centrifugation is the process by which a centrifuge is used to separate components of a complex mixture. By spinning laboratory samples at very high speeds, the components of a given mixture are subjected to centrifugal force, which causes more dense particles to migrate away from the axis of rotation and lighter ones to move toward it. These particles can sediment at the bottom of the tube into what’s...
11:31
A Chromatin Assay for Human Brain Tissue
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Cited by 16 •
2008
Until recently, expression studies on human brain were limited to quantification of RNA or protein. With the chromatin immunoprecipitation techniques described in this paper, it will be possible to map histone methylation and other epigenetic regulators of gene expression in postmortem brain.
10:22
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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Cited by 7 •
2015
We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.
07:51
Pull-down of Calmodulin-binding Proteins
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Cited by 19 •
2012
Calmodulin (CaM) pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses CaM-sepharose beads for efficient and specific analysis of CaM-binding proteins. This provides an important tool to explore CaM signaling in cellular function.
11:02
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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Cited by 227 •
2016
This protocol outlines a fully integrated workflow for characterizing histone post-translational modifications using mass spectrometry (MS). The workflow includes histone purification from cell cultures or tissues, histone derivatization and digestion, MS analysis using nano-flow liquid chromatography and instructions for data analysis. The protocol is designed for completion within 2 - 3 days.
FAQs
Because RCF depends on both rotational speed and the sample’s distance from the rotor axis. Two centrifuges may operate at identical revolutions per minute while placing samples at different radial distances, changing the effective acceleration. Comparing settings by RPM alone can therefore misrepresent the conditions used for a biological separation.
RCF helps exploit differences in the size and density of materials within a sample. Cells, organelles, nucleic acids, proteins, and other particles respond differently during centrifugation because these properties affect their separation behavior. Selecting and reporting the applied force helps researchers obtain and interpret distinct biological fractions.
RCF incorporates the rotor-dependent conditions that RPM alone does not communicate. Reporting it allows researchers to compare the effective acceleration applied to samples even when experiments use different centrifuges or rotor configurations. This improves reproducibility and gives biological protocols a clearer basis for repeating sample-separation conditions.
Researchers can use RCF as the centrifugation condition associated with a planned separation of cells, organelles, nucleic acids, proteins, or other particles. Recording that value with the experiment provides a reproducible reference for the sample-preparation step. It also helps relate the separation condition to later microscopy, molecular biology, or biochemical analyses.
RCF reporting supports workflows in microscopy, molecular biology, biochemistry, and diagnostic research. In each setting, centrifugation may prepare or separate biological material before analysis. Stating the effective acceleration helps distinguish the actual processing condition from the instrument’s rotational speed, making results easier to compare across experiments and laboratories.
Biological investigations often rely on centrifugation to separate complex samples into useful fractions. If protocols provide only rotational speed, differences in rotor geometry can make nominally similar runs apply different accelerations. Reporting RCF reduces that ambiguity, supporting more consistent preparation of cells, organelles, nucleic acids, proteins, and other materials for downstream research.