A Chromatin Assay for Human Brain Tissue

Anouch Matevossian; Schahram Akbarian

doi:10.3791/717

A Chromatin Assay for Human Brain Tissue

JoVE Journal
Biology

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JoVE Journal Biology

A Chromatin Assay for Human Brain Tissue

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11:31 min

March 21, 2008

DOI: 10.3791/717-v

Anouch Matevossian¹, Schahram Akbarian¹

¹Psychiatry, Brudnick Neuropsychiatric Research Institute,University of Massachusetts Medical School

Until recently, expression studies on human brain were limited to quantification of RNA or protein. With the chromatin immunoprecipitation techniques described in this paper, it will be possible to map histone methylation and other epigenetic regulators of gene expression in postmortem brain.

Hi, I'm Amish Matan and I'm from the laboratory of Dr.Sha Mc Barian here in the psychiatry department of UMass Medical School. Today I will show you a procedure for chromatin immunoprecipitation in postmortem human brain tissue. Though the following procedure is not very complicated.

What is most exciting is that until recently expression studies in the human brain were limited to simple quantitation of mRNA and protein. This particular technique will give us a better understanding of the mechanistic basis of gene expression in both normal and diseased human brain. So let's get started To immuno precipitate chromatin.

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