Stomatal Lineage

Stomatal lineage is the developmental pathway through which specialized epidermal cells produce stomata, microscopic pores that regulate gas exchange between plants and the atmosphere. In this process, a stomatal precursor undergoes asymmetric divisions to generate a meristemoid, which may self-renew before differentiating into a guard mother cell; a final symmetric division then forms the paired guard cells surrounding the pore. Cell-to-cell signaling helps control lineage progression and prevents neighboring stomata from forming too closely. Studying stomatal lineage reveals how plants coordinate development with carbon dioxide uptake, water loss, and environmental responses, supporting research in plant biology, stress adaptation, and crop improvement.

Stomatal Lineage - Related Videos

Research

JoVE Journal - Biology

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

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2023

Several commonly used methods are introduced here to study the membrane trafficking events of a plasma membrane receptor kinase. This manuscript describes detailed protocols including the plant material preparation, pharmacological treatment, and confocal imaging setup.

Research

JoVE Journal - Immunology and Infection
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Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

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Cited by 38 •

2010

We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.

Research

JoVE Journal - Biology
Free Sample

Relating Stomatal Conductance to Leaf Functional Traits

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Cited by 39 •

2015

Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.

Education

JoVE Core - Cell Biology

Lineage Commitment

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2023

Commitment is the process whereby stem cells: lose their ability to form all cell types and irreversibly change into a specific type. The multipotent hematopoietic stem cells, (HSCs), differentiate into the multipotent hematopoietic progenitor cells, (HPCs). The HPCs express many lineage-specific cytokine receptors. Each of these receptors binds specific cytokines, activates distinct signaling pathways, and expresses a particular gene set. The HPCs further differentiate to form committed...

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

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Cited by 8 •

2011

This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.

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