Region Of Interest Scan
Region Of Interest Scan - Related Videos
10:10
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
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2017
Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:03
IR Frequency Region: Fingerprint Region
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2024
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1. The...
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
12:03
Differential Scanning Calorimetry
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2023
Source: Danielle N. Beatty and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Differential scanning calorimetry (DSC) is an important measurement to characterize thermal properties of materials. DSC is used primarily to calculate the amount of heat stored in a material as it heats up (heat capacity) as well as the heat absorbed or released during chemical reactions or phase changes. However, measurement of this heat can also lead to...
FAQs
The operator establishes boundaries around the biological feature of interest, creating coordinates that guide where measurements are collected. The instrument then scans, records, or analyzes signals within those limits under selected imaging conditions. This spatial control keeps the dataset aligned with the target area and supports focused examination of localized cellular or tissue features.
Chosen imaging conditions determine how signals are acquired within the selected coordinates. Keeping those conditions appropriate for the specimen helps the scan capture relevant information from cells, tissues, fluorescent markers, or subcellular structures. Consistent conditions also make comparisons of morphology or signal intensity across experimental regions more meaningful.
A targeted scan restricts acquisition to a selected area instead of collecting equivalent information across the whole specimen. This focus can reduce acquisition time and data volume when the biological question concerns a localized feature. It is therefore suited to experiments that prioritize spatially specific morphology or signal measurements.
The selected area may contain cells, tissues, fluorescent markers, or subcellular structures. By concentrating measurement on that area, researchers can inspect localized morphology and signal intensity rather than treating the specimen as a single undifferentiated field. This supports biologically focused analysis when spatial differences within a sample are important.
First, the operator identifies the biological target and draws boundaries around its region. Next, imaging conditions are selected for the intended measurement. The instrument scans or records signals within the defined coordinates, after which the collected data can be analyzed for localized morphology or signal intensity and compared with other experimental regions.
It is useful when microscopy experiments need detailed information from a particular cellular or tissue region without acquiring data from the entire specimen. In quantitative image analysis, the focused dataset can simplify measurements of morphology or signal intensity. The approach also supports monitoring spatial changes in biological samples over an investigation.
Researchers can compare morphological features or signal intensity across experimental regions. Such comparisons may reveal spatial differences in cells, tissues, fluorescent labeling, or subcellular structures. Because each region is defined by coordinates, the analysis can connect measured signals with their location, helping investigators evaluate localized changes within biological samples.