Reporter Mice
Reporter Mice - Related Videos
09:34
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
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Cited by 1 •
2016
This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.
10:30
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
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Cited by 2 •
2017
We describe here a transgenic reporter mouse model to visualize the IL-22-producing cells inside different mouse tissues. This method can be used to track the location of other cytokines or secretary proteins in the mouse.
10:06
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
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Cited by 1 •
2018
We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Cited by 15 •
2015
In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.
01:24
Data Reporting and Recording
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2023
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
FAQs
The regulatory design determines what the mouse reveals. Placing a reporter gene under a selected promoter links the signal to activity controlled by that promoter, whereas Cre-lox recombination provides an alternative way to activate the reporter in cells meeting the chosen genetic condition. Thus, these models answer related but distinct questions about gene regulation or cell identity.
Reporter choice shapes how activity is detected. Fluorescent markers provide an optical readout, luminescent markers produce measurable light, and enzyme-based markers generate a detectable signal through an enzymatic marker. These options do not change the biological target selected by the promoter or recombination system; they determine the form of evidence researchers collect from the model.
Cre-lox-based designs are especially useful when the research question concerns cell history rather than only current gene activity. Recombination can connect reporter activation to a selected cellular or genetic condition, allowing investigators to identify cells associated with that event and follow lineage-related patterns. This makes the model relevant to developmental studies and questions about how cell populations change over time.
Interpretation depends on the biological control built into the model. A promoter aimed at a selected gene reports activity associated with that regulatory region, while a cell-type or pathway design focuses the signal on a defined biological population or process. Choosing the control element therefore determines whether the resulting pattern is read primarily as gene expression, cell identity, or pathway activity.
Designing a reporter mouse begins by specifying the process to observe, such as a gene, cell type, or pathway. Researchers then select a reporter gene and connect it to an appropriate promoter or activation system, including Cre-lox when suitable. The resulting signal is examined in living tissues to relate the molecular target to cellular or organismal behavior.
These models can reveal where and when a selected biological activity occurs in living tissues, rather than limiting analysis to an isolated molecular description. Signal patterns can be compared across development, disease progression, or treatment response to examine how activity relates to changing tissue states. The outcome is a spatial or process-linked view of biology, depending on the model design.
In developmental biology, reporter mice help connect gene regulation with changing tissues and cell populations. In disease research, the same strategy can indicate how a selected pathway or cell type behaves as disease progresses or after treatment. Because the readout links molecular activity to cellular and organismal outcomes, one model can support questions that span multiple biological levels.