Reporter Transgenes
Reporter Transgenes - Related Videos
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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Cited by 14 •
2012
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
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Cited by 27 •
2010
Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.
02:16
Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
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2025
This video demonstrates the generation of neural progenitor cells from non-transgenic and transgenic human pluripotent stem cells (hPSCs). hPSCs are differentiated into neural progenitor cells using a neuronal differentiation medium. While differentiation is induced in non-transgenic cells using small molecules, the transgenic hPSCs are differentiated by adding an antibiotic that induces the promoter for a neuronal differentiation-specific transcription factor.
08:46
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
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Cited by 4 •
2020
A forward genetic screen based on Ca2+ elevation as a read-out leads to identification of genetic components involved in calcium dependent signaling pathways in plants.
04:42
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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2016
Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
FAQs
Regulatory sequences determine when a reporter gene responds by recognizing specific transcription factors or cellular conditions. When those regulatory inputs change, they can drive reporter expression, causing a detectable signal. This arrangement connects pathway activity with an observable output, allowing researchers to examine how gene regulation changes across biological settings rather than measuring regulatory elements in isolation.
The timing of a signal can indicate when a regulatory pathway becomes active, while its location can show which cells, tissues, or regions respond. Signal level provides a measure related to the strength of that activity. Together, these dimensions help researchers map dynamic patterns and connect changes in gene regulation with developmental or cellular outcomes.
Fluorescent proteins and enzymes provide different types of detectable outputs from the same regulatory logic. Fluorescent reporters support visualization of activity in cells, tissues, or organisms, whereas enzyme reporters provide a measurable enzymatic signal. Choosing between them depends on whether the study emphasizes direct visualization or quantitative measurement of reporter expression.
A study begins by selecting regulatory sequences that respond to the transcription factor or cellular condition of interest and linking them to a reporter gene. Researchers then examine the resulting signal in relevant cells, tissues, or organisms, recording its timing, location, or level. These measurements are interpreted as evidence of the associated regulatory activity.
They are useful when researchers need to determine where and when gene-regulatory activity occurs during development. By observing reporter signals across cells or tissues, investigators can map developmental patterns and relate pathway activation to changing biological structures. This approach helps connect regulatory events with phenotypic changes without relying only on a final developmental outcome.
Reporter transgenes can monitor signaling pathways and reveal how genetic or environmental effects alter pathway activity. In disease-mechanism studies, these measurements help relate disrupted regulation to changes in cellular or tissue behavior. In biotechnology, the same principle supports evaluation of promoter function and other regulatory responses, providing measurable information for analyzing engineered biological systems.