Generation of Transgenic <em>C. elegans</em> by Biolistic Transformation

Daniel Hochbaum; Annabel A. Ferguson; Alfred L. Fisher

doi:10.3791/2090

Generation of Transgenic C. elegans by Biolistic Transformation

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Generation of Transgenic C. elegans by Biolistic Transformation

Generation of Transgenic C. elegans by Biolistic Transformation

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10:15 min

August 23, 2010

DOI: 10.3791/2090-v

Daniel Hochbaum¹, Annabel A. Ferguson¹, Alfred L. Fisher¹

¹Department of Medicine,University of Pittsburgh

Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.

The overall goal of this procedure is to generate transgenic worms via biotic or gene gun transfection. First large quantities of worms are grown, then gold particles are coated with DNA, and worms are bombarded, transgenic, progeny, then isolated and rescued, and new transgenic lines are established. Hi, I'm Daniel Owa from the laboratory of Dr.Alfred Fisher in the Medicine Department at the University of Pittsburgh.

Today we'll show you a procedure for generating transgenic worms. We use this procedure to study gene regulation by transcription factors. So let's get started.

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