Retinal Perfusion
Retinal Perfusion - Related Videos
13:02
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
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Cited by 26 •
2011
This article provides a description of how to dissect and record from the isolated retinal preparation in mouse. In particular, we describe how to record light responses from a fluorescently labeled ganglion cell population and subsequently identify and analyze its morphology.
04:35
Light-Evoked Postsynaptic Responses in Neurons of Mouse Retinal Slices
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2025
This video demonstrates the recording of light-evoked post-synaptic responses in a dark-adapted mouse retinal tissue slice preparation. The retinal slice is placed in a recording chamber, and a recording pipette is used to establish electrical continuity with a retinal ganglion cell. Light pulses are applied to generate excitatory post-synaptic potentials in the ganglion cells, which are recorded by the pipette.
07:48
Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
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Cited by 16 •
2015
With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
03:20
Identification of Mouse Retinal Ganglion Cell Subtypes Through Fluorescent Tracing
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2025
Source: Laboissonniere, et. al. Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells. J. Vis. Exp. (2017). This video demonstrates a fluorescent tracing technique to identify retinal ganglion cell (RGC) subtypes in a transgenic mouse retina. The process involves securing the isolated retina in a recording chamber, maintaining cell viability with oxygenated perfusion, and targeting fluorescently labeled RGCs using a pipette with a tracer. By analyzing dendritic morphology and...
FAQs
Retinal perfusion responds to the interaction of vascular pressure, vessel diameter, and local metabolic demand. Pressure provides the driving influence for flow, while changes in vessel caliber affect how much blood can pass through the circulation. Metabolic demand links delivery to tissue needs. Together, these factors influence capillary exchange, oxygen and nutrient availability, and waste removal.
The retinal and choroidal circulations represent coordinated parts of the eye’s blood supply rather than isolated systems. Considering both helps researchers evaluate how blood reaches retinal tissue and how vascular changes may affect tissue maintenance. This distinction matters in biology because an assessment limited to one circulation may not capture the full vascular context relevant to retinal function.
At the capillary level, perfusion links circulating blood with retinal tissue. Exchange allows oxygen and nutrients to reach neural cells while supporting removal of metabolic waste. Because retinal cells require ongoing maintenance for vision, changes in exchange can indicate altered vascular function even when the broader relationship between the retinal and choroidal circulations remains under study.
Researchers can assess retinal perfusion with optical coherence tomography angiography, fluorescein angiography, or laser Doppler methods. These approaches provide ways to examine vascular function in the eye without disrupting the tissue. Using such measurements, investigators can study how blood-flow patterns relate to retinal biology and evaluate vascular changes associated with disease.
These measurements can reveal whether vascular function is altered and help relate blood-flow changes to retinal tissue status. They are useful when researchers examine connections among circulation, neural-cell maintenance, and visual function. Because the techniques are non-disruptive, they support assessment of these relationships without physically disturbing the eye or interrupting the tissue being studied.
In these conditions, researchers measure retinal perfusion to investigate whether altered blood flow contributes to tissue damage or visual loss. The measurements place vascular changes within disease biology rather than treating visual effects alone. This approach is relevant across diabetic retinopathy, glaucoma, retinal vascular occlusions, and other disorders in which circulation may affect retinal health.