Ribosomal Rna Genes
Ribosomal Rna Genes - Related Videos
02:53
Ribosomal RNA Synthesis
0 Views •
2020
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells. Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
02:53
Ribosomal RNA Synthesis
0 Views •
2023
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells. Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Cited by 119 •
2014
Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.
06:21
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
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Cited by 20 •
2013
A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.
13:16
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
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Cited by 26 •
2018
Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.
FAQs
Transcription initially produces precursor rRNAs rather than fully functional molecules. These precursors undergo processing and chemical modification before combining with ribosomal proteins. The sequence of events links gene expression to assembly of the small and large ribosomal subunits, ensuring that the resulting ribosomes can participate in cellular protein production.
Copy-number variation changes how many ribosomal RNA gene copies are present, while transcriptional regulation influences how actively those copies are expressed. Together, these factors can alter the amount of rRNA available for ribosome assembly. Because ribosomes support protein synthesis, changes in their production can influence cell growth.
Conserved sequences provide stable molecular features that can be compared across organisms. Their persistence supports investigations of evolutionary relationships and helps distinguish organisms for taxonomic or microbial studies. At the same time, sequence differences can provide information about how genomes and lineages are organized and related.
Researchers can analyze ribosomal RNA gene sequences as molecular markers for comparing microorganisms. Conserved regions make comparisons possible across diverse samples, while sequence differences help separate related organisms. This approach connects sequence information with microbial identification and taxonomy, offering a genetic basis for organizing and recognizing microbial lineages.
Patterns in these genes can be examined alongside their genomic arrangement and sequence conservation to study genome organization and evolutionary relationships. Such analyses help place organisms within broader lineages and connect molecular features with phylogeny, the study of relationships among organisms. The same information can also contribute to taxonomic classification.
Ribosomal RNA genes are relevant because their expression contributes to the cellular capacity for ribosome production and protein synthesis. During development, changes in this capacity can be studied as part of broader biological regulation. In disease research, altered protein synthesis provides a context for examining how changes in ribosomal RNA gene activity may be involved.