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JoVE Journal
Biology

This content is Open Access.

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
 

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Article DOI: 10.3791/51709
August 29th, 2014

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Summary August 29th, 2014

Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.

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