Rohon-beard Neuron
Rohon-beard Neuron - Related Videos
08:24
Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
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Cited by 3 •
2017
This manuscript describes methods for electrophysiological recordings from spinal neurons of zebrafish embryos and larvae. The preparation maintains neurons in situ and often involves minimum dissection. These methods allow for the electrophysiological study of a variety of spinal neurons, from the initial electrical excitability acquisition through the early larval stages.
00:04
Zebrafish Optogenetics: Activating Genetically Modified Somatosensory Neuron to Study Larval Behavioral Responses
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2023
This video describes optogenetic techniques by activating genetically modified somatosensory neurons and recording elicited behavioral response with a high-speed video camera.
07:25
Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
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Cited by 6 •
2013
Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.
12:47
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
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Cited by 8 •
2014
Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
02:12
Direct Neuronal Reprogramming of Mouse Astrocytes into Neurons
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2025
This video showcases the direct reprogramming of astrocytes expressing transforming factors into neurons. Transformation factors trigger a cascade of molecular events facilitating astrocyte-to-neuron conversion. The process is further supported by supplying specific agents and supplements through a neuronal differentiation medium.
FAQs
Their two axonal projections divide the sensory task between the body surface and spinal cord. Peripheral axons enter the skin, where mechanical stimulation can be detected, while central axons extend longitudinally within the spinal cord. This arrangement links external input to developing sensorimotor circuits before mature sensory pathways, making axon extension and guidance central features of their early function.
Mechanical stimulation of the embryo’s skin activates Rohon-beard sensory signals, which are then transmitted through their central axons toward nascent sensorimotor circuits. The significance is developmental as well as sensory: these neurons provide an early route for environmental information while spinal circuitry is still being assembled. Their activity therefore connects peripheral contact with circuit formation.
Rohon-beard neurons are temporary components of the sensory system because they later undergo programmed cell death or are replaced when dorsal root ganglion neurons establish mature sensory pathways. This transition separates early embryonic sensation from later organization of sensory input. Studying the timing and cellular context of elimination helps explain how developing circuits change as mature pathways emerge.
Several developmental events can be examined separately: neuronal differentiation, peripheral and central axon guidance, synaptic connection formation, and eventual elimination. Together, these stages show how a sensory neuron progresses from specification to incorporation into a circuit and then removal or replacement. Rohon-beard neurons therefore provide a system for relating cell fate, wiring, and developmental remodeling.
Zebrafish and other model organisms allow researchers to observe Rohon-beard neurons during differentiation, axon extension, synaptic connection, and loss. A study can therefore follow multiple stages of the same developmental process rather than examining only a mature endpoint. These observations support investigations of neural development, sensory circuit assembly, and the transition to mature sensory pathways.
Their development includes observable changes in axon growth, guidance, connectivity, and neuronal elimination, all within an early sensory system. These features give researchers a way to examine how sensory neurons and circuits are assembled and later remodeled. The source material identifies Rohon-beard neurons as valuable not only for development and circuit assembly, but also for studying regeneration.