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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
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Neuroscience
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JoVE Journal Neuroscience
Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

DOI:
10.3791/50184-v

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07:25 min

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January 31, 2013

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,
1Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles

Chapters

  • 00:05Title
  • 01:28Prepare Optic Cable
  • 02:36Screen for Transgene Expression
  • 03:27Larvae Mounting and Optical Stimulation Preparation
  • 05:02Activate Single Neurons Using a 473 nm Laser
  • 05:59Results: Optogenetic Activiation of Zebrafish Somatosensory Neurons Using ChEF-tdTomato
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.

Tags

Keywords: OptogeneticsZebrafishSomatosensory NeuronsChEF-tdTomatoNeural CircuitChannelrhodopsinBehaviorTransgeneCREST3Confocal Microscopy473 Nm LaserHigh-speed Video

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