Shear-dependent Adhesion
Shear-dependent Adhesion - Related Videos
03:50
An In Vivo Model to Study Shear-Dependent Bacterial Adhesion
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2026
Source: Claes, J., et al, In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions. J. Vis. Exp. (2015).This video demonstrates an in vivo method for analyzing shear-dependent bacterial adhesion in a live mouse model. Following surgical preparation and catheterization, the mesenteric vasculature is exposed for real-time imaging under a fluorescence microscope. Perfusion with an ionophore and fluorescently labeled bacteria reveals how shear stress triggers...
07:40
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Cited by 3 •
2016
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
13:28
Introducing Shear Stress in the Study of Bacterial Adhesion
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Cited by 9 •
2011
During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.
09:52
Generation of Shear Adhesion Map Using SynVivo Synthetic Microvascular Networks
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Cited by 10 •
2014
Flow chambers used in adhesion experiments typically consist of linear flow paths and require multiple experiments at different flow rates to generate a shear adhesion map. SynVivo-SMN enables the generation of shear adhesion map using a single experiment utilizing microliter volumes resulting in significant savings in time and consumables.
08:51
Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
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Cited by 13 •
2012
This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.
FAQs
Fluid shear changes both how often cells, particles, or microorganisms encounter a surface and how much mechanical force reaches receptor-ligand bonds. At lower or moderate shear, increased encounters or force-dependent bond stabilization can support attachment. As shear rises too far, the same mechanical loading favors bond failure and removal, shifting the system toward detachment.
An increase in collision frequency can raise the number of opportunities for a cell or particle to contact a surface, but contact alone does not guarantee stable adhesion. The outcome also depends on the forces transmitted through receptor-ligand bonds. Separating these effects helps explain why flow can either promote attachment or increase loss.
Some receptor-ligand bonds respond to mechanical loading in a way that makes them more stable within a moderate force range rather than causing immediate detachment. This behavior helps explain how cells can maintain interactions under flow and contributes to processes such as leukocyte rolling, where cells repeatedly engage vessel surfaces while exposed to fluid movement.
Shear-dependent Adhesion links molecular bond mechanics with whole-cell behavior because forces acting at receptor-ligand bonds can alter whether a cell remains attached, rolls, or detaches. This connection allows investigators to interpret visible changes in cell-surface interactions in terms of mechanical events at the bond level, rather than treating adhesion as a fixed property.
A flow-based study can vary fluid shear while observing whether cells, particles, or microorganisms attach, roll, remain associated, or detach from a surface. Researchers can then compare attachment across shear conditions and relate those observations to collision frequency and receptor-ligand bond mechanics. This design directly tests how changing flow transforms surface interactions.
These experiments can provide information about the shear conditions that favor attachment, rolling, or detachment, rather than only measuring adhesion under one unchanging condition. Such results help connect mechanical loading to biological outcomes, including leukocyte-vessel interactions, platelet attachment, and microbial colonization. They also support interpretation of how flow changes interacting cell behavior.
In biology, the approach is relevant to inflammation and thrombosis because leukocyte rolling and platelet attachment occur in flowing environments. It also informs infection research through microbial colonization studies and supports biomaterials research by examining how surfaces interact with cells or microorganisms under flow. Flow-based laboratory models extend these questions in controlled experimental settings.