Sieve Separation
Sieve Separation - Related Videos
01:19
Sieve Analysis and Grading Curves
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2024
Sieve analysis is a method used to determine the particle size distribution of aggregate materials. This process involves the following steps: The required quantity of dried sample of aggregate is weighed. The weighed aggregates are sieved through a set of sieves with square openings arranged in descending order of aperture size, with the largest mesh on top (Column 1 of Table 1). The stack of sieves is shaken or vibrated to facilitate the sorting of particles by size. After sieving for a...
14:16
A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements
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Cited by 24 •
2015
Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.
08:02
Separation of Mixtures via Precipitation
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2023
Source: Laboratory of Dr. Ana J. García-Sáez — University of Tübingen Most samples of interest are mixtures of many different components. Sample preparation, a key step in the analytical process, removes interferences that may affect the analysis. As such, developing separation techniques is an important endeavor not just in academia, but also in industry. One way to separate mixtures is to use their solubility properties. In this short paper, we will deal with aqueous solutions. The...
07:29
Separating Protein with SDS-PAGE
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2023
Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis, or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their size and nothing else. SDS, an anionic detergent, is used to produce an even charge across the length of proteins that have been linearized. By first loading them into a gel made of polyacrylamide and then applying an electric field to the gel, SDS-coated proteins are then separated. The electric field acts as the driving force, drawing the SDS...
08:17
Electrophoretic Separation of Proteins
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Cited by 29 •
2008
In this video, we demonstrate a method for electrophoretic separation of proteins using poly-acrylimide gel electrophoresis (PAGE).
FAQs
Mesh opening size establishes the size threshold for each fraction. Material smaller than the openings can move through, whereas larger material remains above the sieve. Changing the mesh therefore changes which particles or specimens are collected in each portion. In biological work, selecting an appropriate opening helps produce samples suited to microscopy, counting, identification, or later experiments.
Shaking or agitation helps redistribute a mixed sample across the mesh, increasing contact between particles and the openings. Washing can move smaller material through the sieve while helping separate it from larger material or debris. These actions support more effective size sorting, but the resulting fractions still reflect size rather than biological identity, structure, or species alone.
Size fractionation divides a heterogeneous sample into more manageable portions instead of requiring the entire mixture to be examined at once. This can reduce unwanted debris within a selected size range and make specimens or structures easier to prepare for microscopy, counting, and identification. The separated portions also provide a standardized starting material for downstream biological experiments.
Place the mixed sample on a sieve with a defined mesh opening, then apply shaking, agitation, or washing so material interacts with the mesh. Collect the material that passes through separately from the material retained above. Each portion can then be examined or processed according to its size range, allowing researchers to prepare more consistent samples for subsequent analysis.
The approach can be applied to mixed soil, sediment, plant material, and other biological samples containing particles, organisms, or structures of different sizes. By separating these materials into size ranges, researchers can isolate specimens from surrounding material or remove unsuitable debris. This preparation is particularly useful when a sample must be made more uniform before observation or measurement.
Separated size fractions allow researchers to examine material within a narrower and more predictable range. That organization can simplify microscopy, support counting of retained or passing specimens, and assist identification by reducing the amount of unrelated material present. The fractions may also be used as standardized inputs for downstream experiments, linking sample preparation with more consistent biological observations.