Stentor Coeruleus

Stentor coeruleus is a large, trumpet-shaped freshwater ciliate, a single-celled organism that uses coordinated ciliary activity to feed and move. Its body anchors temporarily to surfaces while rows of cilia drive water toward the oral opening; when stimulated, contractile fibers rapidly shorten the cell, and light-sensitive pigments help mediate phototactic responses. Its unusual size, visible morphology, behavioral flexibility, and ability to regenerate missing structures make Stentor coeruleus a useful model for studying cell biology, biomechanics, sensory signaling, and regeneration. Research on this organism shows how a single cell can integrate environmental cues and produce coordinated behavior without a nervous system.

Stentor Coeruleus - Related Videos

Research

JoVE Journal - Biology
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Studying Habituation in Stentor coeruleus

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Cited by 6 •

2023

We introduce a method for quantifying Stentor habituation using a microcontroller board-linked apparatus that can deliver mechanical pulses at a specified force and frequency. We also include methods for assembling the apparatus and setting up the experiment in a way that minimizes external perturbations.

Research

JoVE Journal - Biology
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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

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Cited by 15 •

2013

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

Research

JoVE EoE - Neuropathology

Immunohistochemistry for Identifying the Locus Coeruleus Region in Mouse Brain Sections

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2025

This protocol outlines the use of immunohistochemistry to identify locus coeruleus regions in mouse brain sections. By targeting dopamine β-hydroxylase with green fluorophore-labeled antibodies, norepinephrine-producing neurons are visualized as green fluorescence under a microscope.

Methods for the Study of Regeneration in Stentor

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Cited by 20 •

2018

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

Localization of the Locus Coeruleus in the Mouse Brain

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Cited by 17 •

2019

The locus coeruleus is a small cluster of neurons involved in a variety of physiological processes. Here, we describe a protocol to prepare mouse brain sections for studies of proteins and metals in this nucleus.

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