Stereotaxic Instrument Use
Stereotaxic Instrument Use - Related Videos
11:40
Building An Open-source Robotic Stereotaxic Instrument
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Cited by 18 •
2013
This protocol includes the designs and software necessary to upgrade an existing stereotaxic instrument to a robotic (computer numeric controlled; CNC) stereotaxic instrument for around $1,000 (excluding a drill).
00:08
Survivable Stereotaxic Surgery in Rodents
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Cited by 50 •
2008
The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
04:17
Stereotaxic Viral Injection for Targeted Neuronal Gene Editing in the Mouse Brain
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2025
Source: Fricano-Kugler, C. J., et al. Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection. J. Vis. Exp. (2016)This video demonstrates stereotaxic viral injection for precise neuronal gene editing in the mouse brain. The protocol ensures accurate virus delivery to the dentate gyrus, enabling fluorescent labeling and CRISPR-mediated gene knockout to study neural circuits, gene function, and disease mechanisms in vivo.
09:09
Dissecting the Non-human Primate Brain in Stereotaxic Space
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Cited by 14 •
2009
The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.
FAQs
Three-dimensional coordinates translate a desired anatomical target into a defined position relative to reference landmarks. The instrument then guides a needle, electrode, or other probe along that planned location, helping researchers reach comparable regions across experiments. This coordinate-based approach is especially important when linking a localized intervention or measurement to the function of a particular brain structure.
Stabilization limits movement while a tool is positioned or advanced toward a target. Reducing motion helps preserve the intended relationship between the instrument, the coordinate system, and the anatomical region being studied. As a result, injections, tissue sampling, and electrophysiological recordings can be localized more consistently, which improves comparisons among subjects and strengthens experimental reproducibility.
The tool should match the experimental goal. Needles support delivery of substances, electrodes enable recording of biological activity, and other probes can support tissue sampling or related measurements. Because each tool produces a different type of intervention or data, selection influences whether the study evaluates a treatment response, collects tissue, or examines activity associated with a specific anatomical region.
A typical workflow begins by stabilizing the subject and identifying the anatomical reference framework. Researchers then use defined three-dimensional coordinates to position the instrument and guide the selected tool toward its target. The final step depends on the study objective: delivering a substance, collecting tissue, or recording biological activity. Consistent positioning across these stages supports reliable localization.
This approach is useful when a study requires a localized intervention or measurement within the brain or another anatomical region. Applications include investigating neural circuits, examining behavior, modeling disease mechanisms, and evaluating treatment responses. By connecting a specific regional manipulation or recording with biological outcomes, the method helps researchers relate anatomy to physiology in animal models.
Stereotaxic methods can produce localized substance delivery, region-specific tissue samples, or electrophysiological recordings of biological activity. These outputs allow researchers to compare anatomical regions with physiological function, behavioral effects, disease-related mechanisms, or treatment responses. Consistent targeting also reduces variation in where an intervention or measurement occurs, making findings easier to interpret and reproduce.