Survivable Stereotaxic Surgery in Rodents

Brenda M. Geiger; Lauren E. Frank; Angela D. Caldera-Siu; Emmanuel N. Pothos

doi:10.3791/880

Survivable Stereotaxic Surgery in Rodents

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Biology

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JoVE Journal Biology

Survivable Stereotaxic Surgery in Rodents

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00:08 min

October 6, 2008

DOI: 10.3791/880-v

Brenda M. Geiger¹, Lauren E. Frank¹, Angela D. Caldera-Siu¹, Emmanuel N. Pothos¹

¹Department of Pharmacology and Experimental Therapeutics,Tufts University

The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.

Hi, I am Brenda Geiger from the Laboratory of Emmanuel POTOs in the Department of Pharmacology and Experimental Therapeutics at Tufts University School of Medicine. And I'm Lauren Frank, also from the lab. Today we will demonstrate survivable stereotactic surgery in rodents.

This procedure involves setting up the instruments and preparing the animal for surgery and then performing the stereotaxic surgery. So let's get started. Before starting the procedure, set up the stereotaxic instrument and all the materials needed.

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