Targeted Gene Expression
Targeted Gene Expression - Related Videos
03:27
Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model
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2025
This video demonstrates the tetracycline, or Tet-On, gene expression system in a mouse model. In the presence of doxycycline, a tetracycline derivative, the Tet-On system consisting of reverse tetracycline-controlled transactivator protein becomes activated and expresses the gene of interest.
04:02
Detection of Target Gene Expression in a Bacteria-Infected Mouse Brain
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2026
Source: Zhang, S., et al. Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model. J. Vis. Exp. (2018).This video demonstrates the detection of target gene expression in a bacteria-infected mouse brain. RNA is extracted, treated with a DNA-degrading enzyme, and reverse-transcribed into cDNA. The cDNA is amplified through thermal cycling with gene-specific primers, while a DNA-binding dye binds to the...
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
08:11
Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria
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Cited by 25 •
2013
The goal of these experiments is to generate quantitative time-course data on the growth and gene expression dynamics of attenuated S. typhimurium bacterial colonies growing inside tumors. This video covers tumor cell preparation and implantation, bacteria preparation and injection, whole-animal luminescence imaging, tumor excision, and bacterial colony counting.
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Cited by 15 •
2016
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
FAQs
Promoters and enhancers provide the regulatory logic for targeted gene expression. A promoter is associated with initiating transcription, while an enhancer can help regulate activity in a particular cellular setting. Transcription factors interact with these elements, influencing whether the selected gene is transcribed. Combining these components allows researchers to connect gene activity with a defined cell type or biological condition.
Activation and suppression represent opposite experimental controls. Activation increases transcription of a selected gene, whereas suppression reduces its expression. Comparing both states can help researchers determine how changing that gene affects cellular function. This distinction is especially useful when a study must separate a gene's contribution from effects caused by manipulating unintended cells.
Engineered gene-delivery systems add a control layer beyond regulatory DNA. They can be used with promoters, enhancers, and transcription factors to direct a gene toward selected cells or tissues and to coordinate where expression occurs. This combination is valuable when regulatory control alone would not provide sufficient experimental specificity.
Restricting expression to intended cells matters because gene activity can alter cellular function. Targeting reduces effects in unintended cells, making experimental results easier to interpret. In biology, this precision helps distinguish the consequences of a gene in its relevant tissue or condition from broader changes affecting other parts of the biological system.
A conceptual workflow begins by selecting the gene and identifying the cells, tissues, or biological conditions that should express it. Researchers can then match promoters or enhancers with appropriate transcription factors, or incorporate an engineered gene-delivery system. The resulting design directs when and where transcription occurs, allowing targeted gene expression to be examined in the intended biological context.
Targeted gene expression supports several biology applications. In developmental studies, it can help examine gene function in selected cellular contexts. Researchers also use it to model disease processes, produce desired proteins in chosen cell types, and explore gene-based therapies. These uses connect precise regulation with biological investigation and biotechnology while helping limit effects in unintended cells.