Targeting Sequence
Targeting Sequence - Related Videos
08:38
Targeted DNA Methylation Analysis by Next-generation Sequencing
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Cited by 83 •
2015
Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
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2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
05:17
Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
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2025
This protocol describes an automated, ISO15189-accredited next-generation sequencing workflow for detecting targetable genomic alterations in non-small cell lung cancer (NSCLC) formalin-fixed paraffin-embedded tissues.
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Cited by 15 •
2016
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
15:28
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Cited by 41 •
2009
We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
FAQs
Cellular transport machinery reads a targeting sequence as a localization cue and routes the associated molecule toward the appropriate compartment. The sequence’s specificity helps distinguish destinations rather than merely promoting movement within the cell. This recognition-based logic explains how proteins can reach the endoplasmic reticulum, mitochondria, chloroplasts, or nucleus and function where they are needed.
For proteins entering the endoplasmic reticulum, a signal peptide can recruit the signal recognition particle during synthesis. That interaction connects the newly synthesized protein with the transport pathway leading to the ER. Because the sequence acts while the protein is being produced, it links protein synthesis with intracellular trafficking and supports delivery to the correct cellular location.
Targeting sequences can be made of amino acids or nucleotides, so the relevant recognition process depends on the biological molecule carrying the cue. Protein targeting commonly uses sequence information that directs proteins to organelles, whereas nucleotide-based targeting sequences represent another form of localization signal. This distinction matters when interpreting trafficking studies or designing engineered molecules.
Researchers incorporate targeting sequences into studies of engineered proteins and then examine where those proteins accumulate inside cells. Comparing the observed localization with the intended destination provides information about intracellular trafficking and the specificity of cellular transport. This approach connects sequence information with cellular organization, making targeting sequences useful tools in cell biology research.
Their localization signals allow researchers to control where recombinant or otherwise engineered proteins accumulate within a cell. Directing a protein toward a particular compartment can help investigate its trafficking and organization while connecting its engineered sequence with its cellular destination. This spatial control makes targeting sequences valuable in biotechnology, especially when protein location affects experimental interpretation.
Targeting sequences provide a way to direct therapeutic molecules toward particular cellular locations rather than treating localization as incidental. Their specificity supports the study and engineering of delivery strategies in molecular medicine. Alongside recombinant protein applications, this use highlights how cellular targeting can connect molecular design with the organization and function of living cells.