Tissue Lyophilization
Tissue Lyophilization - Related Videos
02:31
Extracellular Matrix-derived Foam Fabrication via Lyophilization: A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues
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2023
This video describes the method for synthesizing pure ECM-derived foams without the need for chemical crosslinking. These ECM-derived foams find applications as advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
11:14
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
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Cited by 19 •
2015
Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
07:27
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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Cited by 7 •
2016
We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.
08:32
Extraction of Tissue Antigens for Functional Assays
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2012
A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
09:33
Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
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2026
This protocol successfully prepared sufficient high-quality neovascular tissue from glioma samples using laser-capture microdissection for quantitative proteomics analysis of tumor angiogenesis, overcoming the limitations of studies that traditionally focus mainly on angiogenic factors and providing a large-scale proteomics profile of glioma angiogenesis.
FAQs
Sublimation allows ice within the frozen tissue to change directly into vapor, bypassing a liquid-water stage. This pathway matters because liquid water can contribute to structural changes during drying. By controlling the transition under reduced pressure, the method helps produce a dry tissue material while limiting some changes that might occur if water remained liquid during removal.
Reduced surrounding pressure supports the direct conversion of tissue ice into vapor, while controlled heat provides the energy needed for that conversion. These conditions must work together: pressure enables sublimation, and heat drives water removal without simply treating the tissue as a hydrated sample. Their coordinated use determines how effectively the tissue becomes dry.
Rehydration allows the dried material to return to a workable hydrated state for further handling or analysis. Examining the tissue after rehydration can help investigators consider how the drying process affected its structure and biological properties. This makes rehydration useful not only for recovery, but also for evaluating preservation outcomes in biological studies.
The core workflow begins by freezing the tissue, followed by lowering the surrounding pressure and applying controlled heat. Under these conditions, ice is removed as vapor through sublimation. Once drying is complete, the resulting material can be stored or transported in its dry form and may later be rehydrated when further handling or analysis is required.
After water removal, tissue becomes a lightweight, dry material that is easier to store and transport than hydrated tissue. This practical change supports preservation workflows in which specimens or tissue-derived materials must be handled away from the original preparation setting. The material can subsequently be rehydrated, allowing additional handling or analysis after storage.
In biology, this approach supports specimen preservation, preparation of tissue-derived biomaterials, and studies of how drying influences tissue structure and biological properties. Its value therefore extends beyond simple storage. Researchers can use the dried material as a preserved specimen, as a starting material for biomaterial preparation, or as an experimental system for evaluating drying-related changes.