Tissue Permeation
Tissue Permeation - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
0 Views •
2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
0 Views •
Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
05:54
Controlled Odor Mimic Permeation Systems for Olfactory Training and Field Testing
0 Views •
Cited by 1 •
2021
The Controlled Odor Mimic Permeation System is a simple, field-portable, low-cost method of odor delivery for olfactory testing and training. It is constructed of an odorant retained on an adsorbent material and contained inside of a permeable polymer bag allowing controlled release of the odorant vapor over time.
08:45
Whole Organ Tissue Culture
0 Views •
2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
07:48
Working with Human Tissues for Translational Cancer Research
0 Views •
Cited by 4 •
2015
Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.
FAQs
A concentration gradient provides the driving force for diffusion across tissue: molecules move from regions where they are more concentrated toward regions where they are less concentrated. The gradient therefore influences both the direction and potential extent of movement. Examining permeation under different concentration conditions helps researchers evaluate how readily a substance can reach cells through a tissue.
Molecular size and lipid solubility shape how readily a compound passes through tissue. Smaller molecules may encounter less physical restriction, while lipid solubility affects interaction with tissue environments. These properties do not act alone: tissue structure and available aqueous pathways can modify the outcome. Considering all four factors helps explain why different molecules show different permeation behavior.
Tissue architecture can either hinder movement or provide routes through which molecules travel. The availability of aqueous pathways is especially important for substances that can move through water-compatible spaces, whereas the organization of the tissue determines how continuous or restricted those routes are. Consequently, permeation results reflect not only the molecule being studied but also the physical arrangement of the tissue.
Fluid flow can contribute to tissue permeation alongside diffusion down a concentration gradient. Whereas diffusion is associated with differences in concentration, flow can add movement through the tissue environment. Recognizing both contributions helps researchers interpret transport results without attributing every observed change to molecular diffusion alone. This distinction matters when building models of how substances move within living systems.
Such measurements indicate how a substance moves through a tissue and whether it is likely to reach cells beyond a tissue barrier. They can support comparisons among compounds, evaluation of transport behavior, and development of models that predict movement within living systems. The resulting information is useful when interpreting how substances distribute or exchange across biological tissues.
Drug delivery studies use permeation information to examine whether a compound can move through tissue barriers toward its intended cellular location. Toxicity assessment uses the same type of information to consider how substances may distribute within tissues. Together, these applications connect measured transport behavior with decisions about compound performance and potential biological effects.
In living systems, permeation helps frame how nutrients and wastes are exchanged between tissue regions and cells. It also provides context for signaling molecules, whose movement can influence communication across biological spaces. Studying these processes links molecular transport to tissue function, while accounting for barriers and pathways that may limit or enable movement.