Two-kidney One-clip
Two-kidney One-clip - Related Videos
08:21
A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis
0 Views •
Cited by 6 •
2020
A modified 2 kidney 1 clip (2K1C) Goldblatt mouse model was developed using polyurethane tubing to initiate renal artery stenosis, inducing an increase in renin expression and kidney injury. Here, we describe a detailed procedure of preparing and placing the cuff onto the renal artery to generate a reproducible and consistent 2K1C mouse model.
08:19
The Mouse Isolated Perfused Kidney Technique
0 Views •
Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
01:45
Kidney Structure
0 Views •
2019
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries. The renal cortex is the thick outer layer of the kidney. It...
07:29
Intravascular Delivery of Biologics to the Rat Kidney
0 Views •
Cited by 1 •
2016
The administration of drugs for recovery of kidney function requires control of the localization and distribution of the therapeutic compound. Here, we describe in detail a simple technique for intrarenal delivery of drugs in rats. This procedure may be easily performed with no mortality and high reproducibility.
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
0 Views •
2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
FAQs
Lower blood flow to the affected kidney stimulates renin release. This activates the renin-angiotensin-aldosterone system, which promotes vasoconstriction and sodium retention. Together, these responses raise blood pressure beyond the local reduction in renal perfusion, allowing researchers to examine how a kidney senses inadequate blood flow and converts that signal into systemic cardiovascular effects.
Renin initiates hormonal signaling after the kidney detects reduced perfusion. The resulting renin-angiotensin-aldosterone system increases vasoconstriction and supports sodium retention, both of which contribute to elevated blood pressure. Examining this sequence helps connect renal blood-flow changes with endocrine regulation and clarifies why hormonal signaling is central to renovascular hypertension.
The opposite kidney remains normally perfused, providing an important comparison with the clipped kidney. This arrangement helps researchers distinguish effects associated with reduced blood flow in one kidney from broader consequences of sustained hypertension. It also supports investigation of how renal perfusion and kidney function interact when one kidney experiences altered vascular conditions.
Researchers apply a partial constriction to one renal artery while leaving the opposite kidney normally perfused. The constriction reduces blood flow to the selected kidney, creating the renal stimulus that drives renin release and downstream hormonal responses. This arrangement is the essential experimental setup for studying hypertension arising from unequal renal perfusion.
The model enables assessment of sustained high blood pressure together with its cardiovascular and renal effects. Investigators can relate these outcomes to altered renal perfusion, renin-angiotensin-aldosterone signaling, vasoconstriction, and sodium retention. This combined perspective helps evaluate how persistent hypertension affects both circulatory regulation and kidney function rather than measuring blood pressure in isolation.
It is useful when researchers need to study the relationship between renal perfusion, hormonal signaling, and hypertension. The model also provides a framework for investigating cardiovascular and renal consequences of sustained high blood pressure and for evaluating potential antihypertensive therapies. Its value comes from linking a defined renal vascular change with measurable systemic and organ-level effects.