Vacuum Infiltration
Vacuum Infiltration - Related Videos
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
14:24
Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration
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Cited by 4 •
2014
Three-dimensional (3D) microstructured composite beams are fabricated through the directed and localized infiltration of nanocomposites into 3D porous microfluidic networks. The flexibility of this manufacturing method enables the utilization of different thermosetting materials and nanofillers in order to achieve a variety of functional 3D reinforced nanocomposite macroscopic products.
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
04:00
Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells
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2025
This video demonstrates a technique to quantify the relative ratio of macrophages among the total infiltrated immune cell populations in temporal artery biopsy. The immunohistochemical staining of a tissue section affected by autoimmune disease selectively stains the immune cells with specific receptors to visually differentiate them from the rest of the cells.
FAQs
Reduced pressure causes gases trapped within porous biological tissues to expand and escape. When normal pressure is restored, the liquid surrounding the specimen is driven into the spaces previously occupied by those gases. This two-stage pressure change is important because simply immersing a sample may leave internal regions inaccessible, whereas pressure restoration promotes more complete reagent distribution.
Trapped air can prevent a fixative, preservative, stain, or embedding medium from reaching internal spaces uniformly. Regions that receive less reagent may be preserved, colored, or supported less consistently than exposed surfaces. Removing the air improves access throughout the specimen, helping produce more even structural preservation and more reliable microscopy or histological analysis.
The technique is especially relevant when a specimen contains porous tissues or internal spaces that can retain air. Its value depends on whether those spaces need to receive a liquid reagent for preservation, staining, fixation, or embedding. Samples with accessible internal voids can benefit from improved distribution, while the method is less relevant when internal penetration is not needed.
A typical workflow places the biological specimen in the selected liquid, applies reduced pressure to encourage gases inside the tissue to expand and escape, and then restores pressure. The liquid can be a fixative, preservative, staining solution, or embedding medium, depending on the intended analysis. The resulting specimen is then available for microscopy, histology, or another downstream experiment.
Researchers may choose this approach when ordinary exposure to a liquid does not distribute the reagent adequately through a porous specimen. It is useful for preparing samples that require consistent fixation, preservation, staining, or structural support before microscopy and histological analysis. In plant biology, it can also assist with introducing solutions into tissues for imaging and physiological studies.
By improving reagent access to internal tissue spaces, the method can produce more uniformly preserved specimens and better-supported structures for imaging. Depending on the liquid used, it may also prepare tissues for staining or embedding. These outcomes support microscopic and histological examination, while plant studies can use the approach for imaging, physiological investigations, and downstream experiments.